Özer Taha, Başlarlı Özgür, Aktaş Alper, Barış Emre, Ocak Mert
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
Indian J Orthop. 2022 Jun 27;56(8):1424-1430. doi: 10.1007/s43465-022-00673-w. eCollection 2022 Aug.
BACKGROUND/AIM: Denosumab is a human monoclonal immunoglobulin G2 antibody developed from the ovarian cells of Chinese hamsters. We aimed to histomorphometrically and radiologically evaluate the effects of xenografts used with local denosumab on the healing of defect sites using rabbit skulls.
Two 10-mm diameter critical-size defects were created in 16 rabbits. The defect areas were filled with xenografts and xenograft + 3 mg denosumab in the control and denosumab groups (DEN), respectively. We evaluated new bone, residual graft, soft tissue areas, and bone volume in 4- and 8-week study groups.
Histomorphometrically, there were no statistically significant differences between groups at both 4 and 8 weeks regarding residual graft, new bone, and soft tissue area ( > 0.05). The 4-week residual graft control group values were significantly higher than the 8-week values ( < 0.05). The soft tissue area was significantly greater in the 4-week compared with the 8-week DEN group ( < 0.05). The radiologically measured total bone volume was significantly greater in the 8-week specimens than in the 4-week specimens ( < 0.05).
In this study, denosumab used locally with bone grafts, showed no direct effect on new and total bone volume.
背景/目的：地诺单抗是一种从中国仓鼠卵巢细胞中开发的人源单克隆免疫球蛋白G2抗体。我们旨在通过组织形态计量学和放射学方法，使用兔颅骨评估局部使用地诺单抗的异种移植物对缺损部位愈合的影响。
在16只兔子身上制造两个直径10毫米的临界尺寸缺损。在对照组和地诺单抗组（DEN）中，缺损区域分别填充异种移植物和异种移植物 + 3毫克地诺单抗。我们在4周和8周的研究组中评估了新骨、残余移植物、软组织面积和骨体积。
组织形态计量学分析显示，在4周和8周时，两组在残余移植物、新骨和软组织面积方面均无统计学显著差异（> 0.05）。4周时对照组的残余移植物值显著高于8周时的值（< 0.05）。与8周的地诺单抗组相比，4周时的软组织面积显著更大（< 0.05）。放射学测量显示，8周标本的总骨体积显著大于4周标本（< 0.05）。
在本研究中，局部使用骨移植物时联合使用地诺单抗，对新骨和总骨体积无直接影响。
