Oncology and Head and Neck Departments, 58884Hadassah Medical Center and Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.
Int J Biol Markers. 2022 Dec;37(4):360-367. doi: 10.1177/03936155221116388. Epub 2022 Aug 4.
Irradiation, which affects cytokine secretion, is used to treat cancer patients. Cytokine levels have correlations to disease parameters, serving as biomarkers for patients. We aim to explore the effect of irradiation on cytokine production both in vitro (using lymphocytes from healthy donors) and in vivo (using serum levels of head and neck cancer patients following irradiation) and correlating them to mucositis severity/need for percutaneous endoscopic gastroscopy (PEG) tube installation.
Cytokine production by cultured lymphocytes from healthy donors, in vitro, following irradiation of 5 or 10 Gy. In addition, blood from 23 patients with head and neck cancers, irradiated by 60-72G in vivo, were assessed for inflammatory cytokines (tumor necrosis factor (TNF)α, interleukin (IL)-6, IL-8, IL-18), the anti-inflammatory cytokine IL-10, and the general marker sIL-2R. Following radiation, selected patients who were developing mucositis were treated by PEG tube installation. Changes in cytokine levels were studied as predictive biomarkers of response to therapy/PEG tube installation. Cytokine production levels were measured using ELISAs kits.
Irradiation decreased the levels of all tested cytokines, most notably IL-6 and IL-8, proportional to irradiation dose. In patients, increases in cytokine levels, correlated with mucositis severity and potentially the need for PEG tube installation.
Irradiation decreased the levels of all cytokines of healthy lymphocytes in a dose-dependent manner, especially those of IL-6 and IL-8. This study shows a correlation between high and increasing levels of inflammatory cytokines, sIL-2R, plus radiation toxicity and the need for PEG. The reduction of cytokine levels after radiotherapy predicts that PEG will not be required. Thus, our study shows that cytokine changes are predictive biomarkers in head and neck cancer patients.
辐射会影响细胞因子的分泌，被用于治疗癌症患者。细胞因子水平与疾病参数相关，可作为患者的生物标志物。我们旨在探讨辐射对体外（使用健康供者的淋巴细胞）和体内（使用头颈癌患者接受辐射后的血清水平）培养的淋巴细胞产生细胞因子的影响，并将其与黏膜炎严重程度/经皮内镜胃造口术（PEG）管安装的需要相关联。
体外照射 5 或 10 Gy 后，检测健康供者培养的淋巴细胞产生细胞因子的情况。此外，还评估了 23 例接受体内 60-72Gy 照射的头颈癌患者的炎症细胞因子（肿瘤坏死因子（TNF）α、白细胞介素（IL）-6、IL-8、IL-18）、抗炎细胞因子 IL-10 和一般标志物 sIL-2R 的水平。在发生黏膜炎的患者接受 PEG 管安装治疗后，研究了细胞因子水平的变化作为对治疗/PEG 管安装反应的预测生物标志物。使用 ELISA 试剂盒测量细胞因子的产生水平。
辐射降低了所有测试细胞因子的水平，与照射剂量呈比例，以 IL-6 和 IL-8 最为显著。在患者中，细胞因子水平的升高与黏膜炎的严重程度以及可能需要 PEG 管安装相关。
辐射以剂量依赖性方式降低健康淋巴细胞中所有细胞因子的水平，尤其是 IL-6 和 IL-8。本研究显示炎症细胞因子、sIL-2R 加上辐射毒性与需要 PEG 之间存在高且不断增加的水平之间存在相关性。放疗后细胞因子水平的降低预示着不需要 PEG。因此，我们的研究表明细胞因子变化是头颈癌患者的预测生物标志物。
