Department of Biomaterials, Tissue Engineering and Nanotechnology, School of Advanced Technologies in Medicine (ATiM), Isfahan University of Medical Sciences, Isfahan, Iran.
Institute of Polymers, Composites and Biomaterials-National Research Council (IPCB-CNR), Viale J.F. Kennedy 54-Mostra d'Oltremare pad. 20, 80125 Naples, Italy.
Biomater Adv. 2022 Jun;137:212809. doi: 10.1016/j.bioadv.2022.212809. Epub 2022 Apr 19.
Macroporous scaffolds with bioactivity and magnetic properties can be a good candidate for bone regeneration and hyperthermia. In addition, modifying the surface of the scaffolds with biocompatible materials can increase their potential for in vivo applications. Here, we developed a multifunctional nanocomposite MgSiO-CuFeO scaffold for bone regeneration and hyperthermia. The surface of scaffold was coated with various concentrations of poly-3-hydroxybutyrate (P3HB, 1-5% (w/v)). It was observed that 3% (w/v) of P3HB provided a favorable combination of porosity (79 ± 2.1%) and compressive strength (3.2 ± 0.11 MPa). The hyperthermia potential of samples was assessed in the presence of various magnetic fields in vitro. The coated scaffolds showed a lower degradation rate than the un-coated one up to 35 days of soaking in simulated biological medium. Due to the porous and specific morphology of P3HB, it was found that in vitro bioactivity and cell attachment were increased on the scaffold. Moreover, it was observed that the P3HB coating improved the cell viability, alkaline phosphatase activity, and mineralization of the scaffold. Finally, we studied the bone formation ability of the scaffolds in vivo, and implanted the developed scaffold in the rat's femur for 8 weeks. Micro-computed tomography results including bone volume fraction and trabecular thickness exhibited an improvement in the bone regeneration of the coated scaffold compared to the control. The overall results of this study introduce a highly macroporous scaffold with multifunctional performance, noticeable ability in bone regeneration, and hyperthermia properties for osteosarcoma.
具有生物活性和磁性的大孔支架可以成为骨再生和热疗的良好候选物。此外，通过使用生物相容性材料对支架表面进行修饰，可以提高其在体内应用的潜力。在这里，我们开发了一种用于骨再生和热疗的多功能纳米复合 MgSiO-CuFeO 支架。支架的表面涂覆了不同浓度的聚 3-羟基丁酸酯（P3HB，1-5%（w/v））。结果表明，3%（w/v）的 P3HB 提供了一种有利的多孔性（79±2.1%）和抗压强度（3.2±0.11 MPa）的组合。在体外不同磁场下评估了样品的热疗潜力。与未涂层的支架相比，涂层的支架在模拟生物介质中浸泡 35 天内表现出更低的降解率。由于 P3HB 的多孔和特定形态，发现支架上的体外生物活性和细胞附着得到了提高。此外，观察到 P3HB 涂层提高了支架的细胞活力、碱性磷酸酶活性和矿化。最后，我们研究了支架在体内的成骨能力，并将开发的支架植入大鼠股骨 8 周。包括骨体积分数和小梁厚度在内的微计算机断层扫描结果表明，与对照组相比，涂层支架的骨再生能力得到了改善。这项研究的总体结果介绍了一种具有多功能性能、显著骨再生能力和骨肉瘤热疗性能的高度大孔支架。
