Biofilm Biology Laboratory, Centre for Research on Infectious Diseases (CRID), School of Chemical and Biotechnology, SASTRA Deemed University, Tirumalaisamudram, Thanjavur 613 401, Tamil Nadu, India.
Department of Chemistry, School of Chemical and Biotechnology, SASTRA Deemed University, Thanjavur 613 401, Tamil Nadu, India.
Biomater Adv. 2022 Jun;137:212813. doi: 10.1016/j.bioadv.2022.212813. Epub 2022 Apr 20.
Orthopedic implant infections cause a serious threat after implantation. The major source of implant infection is biofilms which are highly tolerant to antibiotics due to the presence of rigid biofilm matrix. Hence to overcome biofilm mediated implant infections, we developed a novel antibiofilm agent, palladium (II) thiazolinyl picolinamide complex (Pd(II)-E). From our study, it was found that Pd(II)-E have profound biofilm inhibition activity and also reduced various virulence factors of Methicillin resistant Staphylococcus aureus (MRSA) including slime synthesis, Phenol soluble modulin (PSM) mediated spreading, Exopolysaccharides production and staphyloxanthin synthesis. Further, Pd(II)-E was coated over the titanium plates which was confirmed using EDX (Energy Dispersive X-Ray) analysis. The Pd(II)-E coated plates were able to prevent the biofilm formation on them which was evident under a Scanning electron microscope (SEM) and several virulent genes were found to be downregulated in the biofilms on the coated titanium plates which confirmed by qPCR. From our findings, it was found that Pd(II)-E coated titanium implants would be an effective alternate approach for preventing biofilm mediated implant infections.
骨科植入物感染在植入后会造成严重威胁。植入物感染的主要来源是生物膜，由于刚性生物膜基质的存在，生物膜对抗生素具有高度耐受性。因此，为了克服生物膜介导的植入物感染，我们开发了一种新型的抗生物膜剂，钯（II）噻唑啉基吡啶甲酰胺配合物（Pd（II）-E）。我们的研究发现，Pd（II）-E 具有很强的生物膜抑制活性，还降低了耐甲氧西林金黄色葡萄球菌（MRSA）的多种毒力因子，包括粘液合成、酚可溶性调节蛋白（PSM）介导的扩散、胞外多糖产生和葡萄球菌黄素合成。此外，Pd（II）-E 被涂覆在钛板上，这一点通过 EDX（能量色散 X 射线）分析得到了证实。Pd（II）-E 涂覆的钛板能够防止生物膜在其上形成，这在扫描电子显微镜（SEM）下很明显，并且在涂覆的钛板上的生物膜中发现了几个毒力基因下调，这通过 qPCR 得到了证实。从我们的研究结果来看，发现 Pd（II）-E 涂覆的钛植入物将是预防生物膜介导的植入物感染的一种有效替代方法。
