Biofilm Biology Laboratory, Anusandhan Kendra II, School of Chemical and Biotechnology, SASTRA University, Tirumalaisamudram, Thanjavur 613 401, Tamil Nadu, India.
School of Chemical and Biotechnology, SASTRA University, Tirumalaisamudram, Thanjavur 613 401, Tamil Nadu, India.
Microb Pathog. 2018 Sep;122:162-173. doi: 10.1016/j.micpath.2018.06.028. Epub 2018 Jun 18.
In the current study we have evaluated the antibiofilm and antivirulent properties of unexplored essential oils (EOs) obtained from Pogostemon heyneanus and Cinnamomum tamala against Methicillin Resistant Staphylococcus aureus (MRSA) strains. The EOs from both the aromatic plants was screened for their ability to prevent biofilm formation and to disrupt preformed biofilms. The efficacy of both the EOs to disrupt the preformed biofilms of various MRSA strains was determined by Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscopy (SEM).The EOs were further able to reduce the Extracellular polymeric substance (EPS) and slime synthesis the two factors of the biofilm assemblage. The EOs was also found to be effective in reducing virulence factors like staphyloxanthin and hemolysin. In silico docking studies were performed for the major components of essential oils and dehydroxysqualene synthase of MRSA which is responsible for the synthesis of staphyloxanthin. The results suggest that (E)-nerolidol showed better binding affinity towards the enzyme. Other compounds have similar binding strengths with the enzyme. Furthermore, the synergistic effect EOs along with the commercially available DNaseI and Marine Bacterial DNase (MBD) showed that the synergistic effect had enhanced biofilm disruption ability. The results show that EOs from P. heyneanus and C. tamala has potential antivirulent and biofilm inhibitory properties against clinical and drug resistant S. aureus strains. The present study highlights the importance of bioprospecting plant based natural products as an alternative for antibiotics owing to the emergence of multi-drug resistant strains.
在目前的研究中，我们评估了从 Pogostemon heyneanus 和 Cinnamomum tamala 获得的未探索的精油 (EO) 对耐甲氧西林金黄色葡萄球菌 (MRSA) 菌株的抗生物膜和抗病毒特性。对两种芳香植物的精油进行了筛选，以评估其防止生物膜形成和破坏已形成生物膜的能力。通过共聚焦激光扫描显微镜 (CLSM) 和扫描电子显微镜 (SEM) 确定了两种精油破坏各种 MRSA 菌株已形成生物膜的功效。精油还能够减少胞外聚合物物质 (EPS) 和粘液合成生物膜组装的两个因素。还发现精油能有效降低金黄色葡萄球菌素和溶血素等毒力因子。对 MRSA 的主要成分和去羟鲨烯合酶进行了计算机对接研究，去羟鲨烯合酶负责合成金黄色葡萄球菌素。结果表明，(E)-橙花叔醇对酶表现出更好的结合亲和力。其他化合物与酶具有相似的结合强度。此外，精油与市售的 DNaseI 和海洋细菌 DNase (MBD) 的协同作用表明，协同作用增强了生物膜破坏能力。结果表明，来自 Pogostemon heyneanus 和 C. tamala 的精油具有针对临床和耐药性金黄色葡萄球菌菌株的抗病毒和生物膜抑制特性。本研究强调了从植物中寻找天然产物作为抗生素替代品的重要性，因为多药耐药菌株的出现。
