State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
State Key Laboratory of Materials-Oriented Chemical Engineering, School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, China.
Biomater Adv. 2022 May;136:212753. doi: 10.1016/j.bioadv.2022.212753. Epub 2022 Mar 18.
The clinical acceleration of skin autogenous healing remains a great challenge, especially in the early stage after injury. In this work, a novel directly injectable hydrogel with high self-adaptability is designed as a provisional matrix to close the apposition of wound edges, using carboxymethyl cellulose and poly (γ-glutamic acid) through Schiff-base reaction. Benefiting from the dynamic covalent cross-linking structure, the functional biodegradable hydrogels are easy to prepare (gel time 5-180 s), demonstrating adequate mechanical strength (40-120 kPa), anti-fatigue abilities, and rapid self-healing (5-10 min at skin defect). Furthermore, the hydrogels exhibit biocompatibility and proliferation-promoting activity with murine fibroblasts. In the full-thickness dermal animal models, it significantly promoted collagen deposition, skin-function restoration, and VEGF expression. This hydrogel shows potential as a dressing available for skin regeneration during the healing of dermal injuries.
皮肤自体愈合的临床加速仍然是一个巨大的挑战，特别是在受伤后的早期阶段。在这项工作中，设计了一种具有高自适应性的新型可直接注射水凝胶作为临时基质，以闭合伤口边缘的贴合，使用羧甲基纤维素和聚（γ-谷氨酸）通过席夫碱反应。得益于动态共价交联结构，功能性可生物降解水凝胶易于制备（凝胶时间 5-180 s），具有足够的机械强度（40-120 kPa）、抗疲劳能力和快速自修复能力（在皮肤缺损处 5-10 min）。此外，水凝胶对小鼠成纤维细胞表现出良好的生物相容性和增殖促进活性。在全厚度真皮动物模型中，它显著促进了胶原蛋白沉积、皮肤功能恢复和 VEGF 表达。这种水凝胶有望成为一种用于皮肤再生的敷料，可用于皮肤损伤的愈合过程。
