Gościcka T, Walencka M
Arch Immunol Ther Exp (Warsz). 1986;34(5-6):489-97.
The level of cells expressing Fc receptor and their ability to antibody mediated cytotoxicity was determined in this study. The adherent cells fraction isolated from blood leukocytes, splenocytes and peritoneal cells of Listeria monocytogenes infected Balb/c mice were rosetted with Ab-SRBC or SRBC. Moreover, blood leukocytes, splenocytes and peritoneal cells were incubated with Ab-51Cr SRBC and 51Cr SRBC as target cells and their cytotoxic activity was assessed by the per cent of specific 51Cr release. The results indicate that in the Listeria infected organisms the level of cells rosetting with Ab-SRBC is greatly increased particularly in peritoneal cells between the 3rd and 7th day of infection i.e. in the period of intensive production of macrophages. Only peritoneal cells exhibit the antibody dependent cytotoxicity. However, the intensity of this cytotoxicity does not correlate with the level of cells expressing Fc receptor.
本研究测定了表达Fc受体的细胞水平及其抗体介导的细胞毒性能力。从单核细胞增生李斯特菌感染的Balb/c小鼠的血液白细胞、脾细胞和腹腔细胞中分离出的贴壁细胞部分，用抗体包被的绵羊红细胞（Ab-SRBC）或绵羊红细胞（SRBC）进行玫瑰花结形成试验。此外，将血液白细胞、脾细胞和腹腔细胞与抗体-51铬标记的绵羊红细胞（Ab-51Cr SRBC）和51铬标记的绵羊红细胞（51Cr SRBC）作为靶细胞一起孵育，并通过特异性51铬释放百分比评估其细胞毒性活性。结果表明，在李斯特菌感染的机体中，与Ab-SRBC形成玫瑰花结的细胞水平显著增加，特别是在感染第3天至第7天的腹腔细胞中，即在巨噬细胞大量产生的时期。只有腹腔细胞表现出抗体依赖性细胞毒性。然而，这种细胞毒性的强度与表达Fc受体的细胞水平无关。
