Kempf C, Kohler U, Michel M R, Koblet H
Arch Virol. 1987;95(1-2):111-22. doi: 10.1007/BF01311338.
Infection of Aedes albopictus cells with Semliki Forest virus (SFV) leads to polykaryocyte formation below pH 6.2. This syncytium formation is accompanied by a decrease of the cellular ATP level. Addition of inhibitors of oxidative phosphorylation leads to a rapid, total depletion of ATP in infected cells at pH 6 and results in an inhibition of polykaryocyte formation. However, when cells were exposed for only a few minutes to pH 6 in the presence of the inhibitors and then kept at pH 7.2, the ATP level partially recovered to values sufficient for syncytium formation. Similar results were obtained after ATP depletion induced by 2-deoxyglucose. Thus, it can be concluded that SFV-induced syncytium formation is an ATP-dependent event.
用辛德毕斯病毒（SFV）感染白纹伊蚊细胞会导致在pH 6.2以下形成多核细胞。这种多核体形成伴随着细胞ATP水平的降低。添加氧化磷酸化抑制剂会导致感染细胞在pH 6时ATP迅速完全耗尽，并抑制多核细胞的形成。然而，当细胞在抑制剂存在下仅暴露于pH 6几分钟，然后保持在pH 7.2时，ATP水平部分恢复到足以形成多核体的值。用2-脱氧葡萄糖诱导ATP耗尽后也得到了类似的结果。因此，可以得出结论，SFV诱导的多核体形成是一个依赖ATP的事件。
