Department of Plant Pathology, University of Agriculture Faisalabad, Faisalabad, Pakistan.
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
PLoS One. 2021 Sep 16;16(9):e0256449. doi: 10.1371/journal.pone.0256449. eCollection 2021.
Mungbean yellow mosaic virus (MYMV) is an important constraint in successful production of mungbean (Vigna radiata L.) in many countries, including Pakistan. The MYMV spreads by insect vector whitefly (Bemisia tabaci Gennadius). The use of resistant cultivars is the most effective management tactics for MYMV. Twenty mungbean varieties/lines were screened against insect vector of MYMV under field condition in the current study. Resistance levels for varieties/lines were assessed through visual scoring of typical disease symptoms. Furthermore, the impacts of two insecticides 'Imidacloprid' and 'Thiamethoxam' and two plant extracts, i.e., neem (Azadirachta indica), and Eucalyptus (Eucalyptus camaldulensis) were tested on the suppression of whitefly. Field screening indicated that none of the tested varieties/lines proved immune/highly resistant, while significant variations were recorded among varieties/lines for resistance level. All varieties/lines were systemically infected with MYMV. The varieties 'AARI-2006' and 'Mung-14043' were considered as resistant to MYMV based on visual symptoms and the lowest vector population. These varieties were followed by 'NM-2006' and 'NL-31', which proved as moderately resistant to MYMV. All remaining varieties/lines were grouped as moderately to highly susceptible to MYMV based on visual symptoms' scoring. These results revealed that existing mungbean germplasm do not possess high resistance level MYMV. However, the lines showing higher resistance in the current study must be exploited in breeding programs for the development of resistant mungbean varieties/lines against MYMV. Imidacloprid proved as the most effective insecticide at all concentrations to manage whitefly population. Therefore, use of the varieties with higher resistance level and spraying Imidacloprid could lower the incidence of MYMV.
菜豆黄花叶病毒（MYMV）是包括巴基斯坦在内的许多国家成功种植绿豆（Vigna radiata L.）的重要制约因素。MYMV 通过昆虫介体烟粉虱（Bemisia tabaci Gennadius）传播。使用抗性品种是防治 MYMV 的最有效管理策略。本研究在田间条件下对 20 个绿豆品种/系进行了抗虫介体筛选。通过观察典型病害症状对品种/系进行抗性水平评估。此外，还测试了两种杀虫剂（氯吡虫啉和噻虫嗪）和两种植物提取物（印楝和桉树）对粉虱的抑制作用。田间筛选表明，没有一种测试品种/系表现出免疫/高抗，而品种/系之间的抗性水平存在显著差异。所有品种/系均被 MYMV 系统感染。根据视觉症状和最低媒介种群，品种 'AARI-2006' 和 'Mung-14043' 被认为对 MYMV 具有抗性。这些品种随后是 'NM-2006' 和 'NL-31'，它们对 MYMV 表现出中度抗性。根据视觉症状评分，所有其余品种/系均被归类为对 MYMV 中度至高度敏感。这些结果表明，现有的绿豆种质资源不具有高水平的 MYMV 抗性。然而，在当前研究中表现出较高抗性的品系必须在育种计划中加以利用，以培育出对 MYMV 具有抗性的绿豆品种/系。氯吡虫啉在所有浓度下均被证明是管理粉虱种群最有效的杀虫剂。因此，使用具有较高抗性水平的品种并喷洒氯吡虫啉可以降低 MYMV 的发病率。
