Animal & Plant Inspection and Quarantine Technology Center of Shenzhen Customs District P.R. China, Shenzhen, China.
Shenzhen Key Laboratory for Research & Development on Detection Technology of Alien Pests, Shenzhen Academy of Inspection and Quarantine, Shenzhen, China.
PLoS One. 2022 Aug 5;17(8):e0272435. doi: 10.1371/journal.pone.0272435. eCollection 2022.
In this study, we sequenced a bacteria isolate Pandoraea sp. 892iso isolated from a Phytophthora rubi strain which is an important plant pathogenic oomycete, identified through genome and combined the data with existing genomic data from other 28 the genus of Pandoraea species. Next, we conducted a comparative genomic analysis of the genome structure, evolutionary relationships, and pathogenic characteristics of Pandoraea species. Our results identified Pandoraea sp. 892iso as Pandoraea sputorum at both the genome and gene levels. At the genome level, we carried out phylogenetic analysis of single-copy, gene co-linearity, ANI (average nucleotide identity) and AAI (average amino acid identity) indices, rpoB similarity, MLSA phylogenetic analysis, and genome-to-genome distance calculator calculations to identify the relationship between Pandoraea sp. 892iso and P. sputorum. At the gene level, the quorum sensing genes ppnI and ppnR and the OXA-159 gene were assessed. It is speculated that Pandoraea sp. 892iso is the endosymbiont of the Oomycetes strain of Phytophthora rubi.
在这项研究中，我们对从一种重要的植物病原卵菌疫霉属菌中分离出的 Pandoraea sp. 892iso 进行了测序，该菌是通过基因组鉴定的，并结合了来自其他 28 种 Pandoraea 属物种的现有基因组数据。接下来，我们对 Pandoraea 物种的基因组结构、进化关系和致病特性进行了比较基因组分析。我们的研究结果在基因组和基因水平上均将 Pandoraea sp. 892iso 鉴定为 Pandoraea sputorum。在基因组水平上，我们进行了单拷贝、基因共线性、ANI（平均核苷酸同一性）和 AAI（平均氨基酸同一性）指数、rpoB 相似性、MLSA 系统发育分析和基因组到基因组距离计算器计算，以确定 Pandoraea sp. 892iso 与 P. sputorum 之间的关系。在基因水平上，评估了群体感应基因 ppnI 和 ppnR 以及 OXA-159 基因。推测 Pandoraea sp. 892iso 是 Phytophthora rubi 卵菌菌株的内共生体。
