Department of Agricultural Chemistry, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Organic Agriculture Division, National Academy of Agricultural Science (formerly National Institute of Agricultural Science and Technology), Rural Development Administration, Suwon 441-707, Republic of Korea.
Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):21-26. doi: 10.1099/ijs.0.012823-0. Epub 2009 Jul 30.
A facultatively chemolithoautotrophic, thiosulfate-oxidizing, Gram-negative, aerobic, motile, rod-shaped bacterial strain, designated ATSB16(T), was isolated from rhizosphere soils of sesame (Sesamum indicum L.). 16S rRNA gene sequence analysis demonstrated that this strain was closely related to Pandoraea pnomenusa LMG 18087(T) (96.7 % similarity), P. pulmonicola LMG 18016(T) (96.5 %), P. apista LMG 16407(T) (96.2 %), P. norimbergensis LMG 18379(T) (96.1 %) and P. sputorum LMG 18819(T) (96.0 %). Strain ATSB16(T) shared 96.0-96.4 % sequence similarity with four unnamed genomospecies of Pandoraea. The major cellular fatty acids of the strain ATSB16(T) were C(17 : 0) cyclo (33.0 %) and C(16 : 0) (30.6 %). Q-8 was the predominant respiratory quinone. The major polar lipids were phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified aminophospholipids. Hydroxyputrescine and putrescine were the predominant polyamines. The genomic DNA G+C content of the strain was 64.0 mol%. On the basis of the results obtained from this study, strain ATSB16(T) represents a novel species of the genus Pandoraea, for which the name Pandoraea thiooxydans sp. nov. is proposed. The type strain is ATSB16(T) (=KACC 12757(T) =LMG 24779(T)).
一株兼性化能自养、硫代硫酸盐氧化、革兰氏阴性、需氧、运动、杆状细菌菌株，被命名为 ATSB16(T)，从芝麻（Sesamum indicum L.）根际土壤中分离得到。16S rRNA 基因序列分析表明，该菌株与 Pandoraea pnomenusa LMG 18087(T)（96.7%相似度）、P. pulmonicola LMG 18016(T)、P. apista LMG 16407(T)、P. norimbergensis LMG 18379(T) 和 P. sputorum LMG 18819(T) 密切相关（相似度分别为 96.5%、96.2%、96.1%和 96.0%）。菌株 ATSB16(T) 与 Pandoraea 的四个未命名基因组种的序列相似度为 96.0-96.4%。菌株 ATSB16(T) 的主要细胞脂肪酸为 C(17:0) 环（33.0%）和 C(16:0)（30.6%）。Q-8 是主要的呼吸醌。主要的极性脂类是磷脂酰甲醇乙醇胺、双磷脂酰甘油、磷脂酰乙醇胺和两种未鉴定的氨基磷脂。腐胺和尸胺是主要的多胺。该菌株的基因组 DNA G+C 含量为 64.0mol%。基于本研究的结果，菌株 ATSB16(T) 代表 Pandoraea 属的一个新种，建议将其命名为 Pandoraea thiooxydans sp. nov.。模式菌株为 ATSB16(T)（=KACC 12757(T)=LMG 24779(T)）。
