Grup d'Enginyeria de Materials (Gemat), Institut Químic de Sarrià (IQS), Universitat Ramon Llull (URL), Via Augusta 390, 08017 Barcelona, Spain.
Grup d'Enginyeria de Materials (Gemat), Institut Químic de Sarrià (IQS), Universitat Ramon Llull (URL), Via Augusta 390, 08017 Barcelona, Spain.
Colloids Surf B Biointerfaces. 2022 Oct;218:112745. doi: 10.1016/j.colsurfb.2022.112745. Epub 2022 Aug 1.
Despite the great knowledge achieved in the field of extracellular vesicles (EVs), the short lifetime of EVs liquid formulation still hampers the transfer of EVs technology to clinical applications. In this context, freeze-dried EVs would be advantageous thanks to the enhanced stability of solid formulations. Although some previous attempts have already been reported, the efficiency of EVs lyophilization methodologies used remains insufficient, and the characterization of the resulting EVs is still incomplete. The current work aims to describe an alternative and easy-to-be-applied methodology for EVs lyophilization. The use of sucrose as lyoprotectant at 8.5%wt improved the cryopreservation efficiency. After the subsequent cycles of freeze-drying, properties such as size, morphology, purity, EVs specific markers, biocompatibility and the maintenance of their functionality were confirmed in freeze-dried EVs samples. To sum up, we have designed a methodology for the lyophilization of extracellular vesicles that enables the preservation of the physicochemical properties and functionality of EVs.
尽管在细胞外囊泡（EVs）领域取得了巨大的知识成就，但 EVs 液体制剂的短寿命仍然阻碍了 EVs 技术向临床应用的转移。在这种情况下，冻干 EVs 将具有优势，因为固体制剂的稳定性得到了增强。尽管已经有一些先前的尝试，但所使用的 EVs 冻干方法的效率仍然不足，并且对得到的 EVs 的表征仍然不完整。本工作旨在描述一种替代的、易于应用的 EVs 冻干方法。使用 8.5%wt 的蔗糖作为冷冻保护剂可提高冷冻保存效率。在随后的冻干循环后，冻干 EVs 样品的大小、形态、纯度、EVs 特异性标志物、生物相容性和功能维持等特性得到了确认。总之，我们设计了一种用于冻干细胞外囊泡的方法，能够保持 EVs 的物理化学性质和功能。
