Grover G J, Weiss H R
Basic Res Cardiol. 1987 Jan-Feb;82(1):57-65. doi: 10.1007/BF01907053.
The purpose of this study was to determine whether depletion of serum complement can decrease the severity of an ischemic episode by improving regional O2 supply and consumption parameters in the ischemic region of the heart. Fourteen anesthetized dogs with serum complement intact or depleted (100 U/kg cobra venom factor given 8 hrs before) were subjected to left anterior descending coronary artery (LAD) occlusion for 6 hrs. Myocardial blood flows were determined before and 6 hrs after LAD occlusion using radioactive microspheres. Regional arterial and venous O2 saturations were determined using microspectrophotometry. In control animals, flow decreased from 122 +/- 42 to 13 +/- 14 ml/min/100 g (mean +/- SD) in the occluded LAD region. With complement depletion, LAD occlusion resulted in a flow reduction in the ischemic region (38 +/- 29 ml/min/100 g), but to a lesser degree than seen in the same region in control animals, especially in the subendocardium. O2 consumption was decreased in the ischemic region of both treatment groups, though O2 consumption was higher in this region in complement depleted animals compared to the values in control animals. The O2 supply/consumption ratio was decreased similarly in the ischemic region of control and complement depleted groups. Thus, with complement depletion, flow to the ischemic zone was improved but this region was still flow restricted. The flow increase during complement depletion was sufficient to allow an increased O2 utilization in the ischemic region.
本研究的目的是确定血清补体的耗竭是否能通过改善心脏缺血区域的局部氧供应和消耗参数来降低缺血发作的严重程度。14只血清补体完整或已耗竭（8小时前给予100 U/kg眼镜蛇毒因子）的麻醉犬，接受左冠状动脉前降支（LAD）闭塞6小时。使用放射性微球测定LAD闭塞前和闭塞6小时后的心肌血流量。使用显微分光光度法测定局部动脉和静脉血氧饱和度。在对照动物中，闭塞的LAD区域血流量从122±42降至13±14 ml/min/100 g（平均值±标准差）。补体耗竭后，LAD闭塞导致缺血区域血流量减少（38±29 ml/min/100 g），但程度低于对照动物同一区域，尤其是心内膜下区域。两个治疗组的缺血区域氧消耗均降低，不过与对照动物相比，补体耗竭动物该区域的氧消耗更高。对照和补体耗竭组的缺血区域氧供应/消耗比同样降低。因此，补体耗竭时，缺血区血流量得到改善，但该区域仍存在血流受限。补体耗竭期间血流量的增加足以使缺血区域的氧利用增加。
