使用羧酸酐进行核蛋白颗粒结构研究时的陷阱。
Pitfalls in the use of carboxylic acid anhydrides for structural studies of nucleoprotein particles.
作者信息
Nieto M A, Palacián E
出版信息
Biochem J. 1987 Jan 15;241(2):621-3. doi: 10.1042/bj2410621.
Abstract
During modification of protein amino groups with carboxylic acid anhydrides, these reagents cause a fall in pH, which can be prevented by addition of base. Although unmodified nucleosomal particles are not affected by the local transient changes in pH induced by the base (NaOH) added to prevent a fall in pH during modification, the nucleosomal particles modified by acetic anhydride are dissociated, with release of single-stranded DNA.
摘要
在用羧酸酐修饰蛋白质氨基的过程中，这些试剂会导致pH值下降，可通过添加碱来防止这种情况。虽然未修饰的核小体颗粒不受为防止修饰过程中pH值下降而添加的碱（NaOH）引起的局部短暂pH值变化的影响，但经乙酸酐修饰的核小体颗粒会发生解离，并释放出单链DNA。
相似文献
1
Pitfalls in the use of carboxylic acid anhydrides for structural studies of nucleoprotein particles.使用羧酸酐进行核蛋白颗粒结构研究时的陷阱。
Biochem J. 1987 Jan 15;241(2):621-3. doi: 10.1042/bj2410621.
2
Dissociation of single-stranded DNA from nucleosomes following modification with acetic anhydride.用乙酸酐修饰后，单链DNA从核小体上解离。
Biochem Biophys Res Commun. 1984 Jun 29;121(3):907-14. doi: 10.1016/0006-291x(84)90763-0.
3
Interaction of acetic anhydride with di- and tri-carboxylic acids in aqueous solution.乙酸酐与二元和三元羧酸在水溶液中的相互作用。
J Pharm Sci. 1966 Nov;55(11):1200-4. doi: 10.1002/jps.2600551106.
4
Solvolytic reactions of cyclic anhydrides in anhydrous acetic acid.环状酸酐在无水乙酸中的溶剂解反应。
J Pharm Sci. 1975 Nov;64(11):1759-65. doi: 10.1002/jps.2600641105.
5
[Action of acetic anhydride on yeast ribonuleic acids in dimethylformamide].[乙酸酐在二甲基甲酰胺中对酵母核糖核酸的作用]
Biochim Biophys Acta. 1968 Aug 23;166(1):16-28.
6
Reactivity of tobacco mosaic virus and its protein toward acetic anhydride.烟草花叶病毒及其蛋白质对乙酸酐的反应活性。
Biochemistry. 1967 Sep;6(9):2740-5. doi: 10.1021/bi00861a014.
7
Assembly of protein and nucleoprotein particles from extracted tobacco rattle virus protein and RNA.
Science. 1969 May 2;164(3879):559-60. doi: 10.1126/science.164.3879.559.
8
Scission of Escherichia coli deoxyribonucleic acid in alkali.
Biochemistry. 1973 Apr 24;12(9):1772-4. doi: 10.1021/bi00733a017.
9
Acylation of phenol by cyclic and acyclic anhydrides in anhydrous acetic acid.在无水乙酸中，通过环状酸酐和链状酸酐对苯酚进行酰化反应。
J Pharm Sci. 1975 Nov;64(11):1766-70. doi: 10.1002/jps.2600641106.
10
Dicarboxylic acid anhydrides as dissociating agents of protein-containing structures.作为含蛋白质结构解离剂的二羧酸酐
Mol Cell Biochem. 1990 Sep 21;97(2):101-11. doi: 10.1007/BF00221051.
引用本文的文献
1
Disassembly and reconstitution of yeast 60S ribosomal subunits.酵母60S核糖体亚基的拆卸与重组
Mol Cell Biochem. 1989 Mar 16;86(1):55-63. doi: 10.1007/BF00231689.
2
Reconstitution of rat liver 60S ribosomal subunits following disassembly by dimethylmaleic anhydride.经顺丁烯二酸酐拆解后大鼠肝脏60S核糖体亚基的重组
Mol Cell Biochem. 1990 Feb 9;92(2):159-67. doi: 10.1007/BF00218133.
3
Dicarboxylic acid anhydrides as dissociating agents of protein-containing structures.作为含蛋白质结构解离剂的二羧酸酐
Mol Cell Biochem. 1990 Sep 21;97(2):101-11. doi: 10.1007/BF00221051.
本文引用的文献
1
Partial reconstitution of active eukaryotic ribosomes following dissociation with dimethylmaleic anhydride.用顺丁烯二酸二甲酯解离后活性真核核糖体的部分重建。
J Biol Chem. 1982 Jun 10;257(11):6477-80.
2
Dissociation of single-stranded DNA from nucleosomes following modification with acetic anhydride.用乙酸酐修饰后，单链DNA从核小体上解离。
Biochem Biophys Res Commun. 1984 Jun 29;121(3):907-14. doi: 10.1016/0006-291x(84)90763-0.
3
Effects of temperature and pH on the regeneration of the amino groups of ovalbumin after modification with citraconic and dimethylmaleic anhydrides.温度和pH对用柠康酸酐和二甲基马来酸酐修饰后卵清蛋白氨基再生的影响。
Biochim Biophys Acta. 1983 Dec 12;749(2):204-10. doi: 10.1016/0167-4838(83)90254-6.
4
Rearrangement of nucleosomal components by modification of histone amino groups. Structural role of lysine residues.
Biochemistry. 1984 Sep 11;23(19):4280-4. doi: 10.1021/bi00314a004.
5
Modification of histone binding in calf thymus chromatin and in the chromatin-protamine complex by acetic anhydride.用乙酸酐对小牛胸腺染色质及染色质-鱼精蛋白复合物中组蛋白结合的修饰作用。
Biochemistry. 1976 May 18;15(10):2041-6. doi: 10.1021/bi00655a003.
6
Histones H2a, H2b, H3, and H4 form a tetrameric complex in solutions of high salt.组蛋白H2a、H2b、H3和H4在高盐溶液中形成四聚体复合物。
Cell. 1975 Sep;6(1):85-110. doi: 10.1016/0092-8674(75)90077-x.
7
Activation of chromatin by acetylation of histone side chains.通过组蛋白侧链乙酰化激活染色质。
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3937-41. doi: 10.1073/pnas.73.11.3937.
8
Analysis of DNA of isolated chromatin subunits.分离染色质亚基的DNA分析
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3978-82. doi: 10.1073/pnas.72.10.3978.
9
Reversible modification of Escherichia coli ribosomes with 2,3-dimethylmaleic anhydride. A new method to obtain protein-deficient ribosomal particles.用2,3-二甲基马来酸酐对大肠杆菌核糖体进行可逆修饰。一种获得蛋白质缺陷型核糖体颗粒的新方法。
Biochemistry. 1979 Jul 24;18(15):3219-23. doi: 10.1021/bi00582a005.
10
Physical properties of chemically acetylated rat liver chromatin.化学乙酰化大鼠肝脏染色质的物理性质
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3244-8. doi: 10.1073/pnas.74.8.3244.
Zotero 插件