通过组蛋白侧链乙酰化激活染色质。
Activation of chromatin by acetylation of histone side chains.
作者信息
Marushige K
出版信息
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3937-41. doi: 10.1073/pnas.73.11.3937.
Abstract
The ability of calf thymus chromatin to support DNA-dependent RNA synthesis is markedly increased by modification of the histones with acetic anhydride. The extent of chemical acetylation of histones H2a, H2b, H3, and H4 required for the increase of template activity of the chromatin is within that which can be achieved enzymatically in the cell. The evidence suggests that the acetylation of histone side chains stimulates the rate of chain elongation during transcription of chromatin.
摘要
用乙酸酐修饰组蛋白后，小牛胸腺染色质支持依赖DNA的RNA合成的能力显著增强。染色质模板活性增加所需的组蛋白H2a、H2b、H3和H4的化学乙酰化程度，在细胞中通过酶促反应所能达到的范围内。有证据表明，组蛋白侧链的乙酰化可刺激染色质转录过程中的链延伸速率。
相似文献
1
Activation of chromatin by acetylation of histone side chains.通过组蛋白侧链乙酰化激活染色质。
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3937-41. doi: 10.1073/pnas.73.11.3937.
2
Transcription by eukaryotic RNA polymerases A and B of chromatin assembled in vitro.真核生物RNA聚合酶A和B对体外组装染色质的转录。
Eur J Biochem. 1979 Aug 1;98(2):317-27. doi: 10.1111/j.1432-1033.1979.tb13191.x.
3
Acetylation of histone H2A.H2B dimers facilitates transcription.组蛋白H2A.H2B二聚体的乙酰化促进转录。
Biochem Biophys Res Commun. 1995 May 16;210(2):409-16. doi: 10.1006/bbrc.1995.1676.
4
Effects of selective extraction of histones on template activities of chromatin by use of exogenous DNA and RNA polymerases.利用外源DNA和RNA聚合酶选择性提取组蛋白对染色质模板活性的影响。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1973-5. doi: 10.1073/pnas.70.7.1973.
5
Transcriptional properties of oligonucleosomal templates containing acetylated (H3-H4)2 tetramers.
Biochem Biophys Res Commun. 1995 Aug 4;213(1):232-8. doi: 10.1006/bbrc.1995.2121.
6
Histone release during transcription: acetylation stabilizes the interaction of the H2A-H2B dimer with the H3-H4 tetramer in nucleosomes that are on highly positively coiled DNA.转录过程中的组蛋白释放：乙酰化作用可稳定H2A - H2B二聚体与位于高度正超螺旋DNA上的核小体中H3 - H4四聚体之间的相互作用。
Biochemistry. 2005 Dec 13;44(49):16351-64. doi: 10.1021/bi050876o.
7
In vitro transcription of chromatin containing histones hyperacetylated in vivo.对体内组蛋白发生超乙酰化的染色质进行体外转录。
Nucleic Acids Res. 1980 Sep 25;8(18):4201-19. doi: 10.1093/nar/8.18.4201.
8
Histone release during transcription: NAP1 forms a complex with H2A and H2B and facilitates a topologically dependent release of H3 and H4 from the nucleosome.转录过程中的组蛋白释放：核小体组装蛋白1（NAP1）与H2A和H2B形成复合物，并促进H3和H4从核小体中进行拓扑依赖性释放。
Biochemistry. 2004 Mar 9;43(9):2359-72. doi: 10.1021/bi035737q.
9
Transcription of histone-covered T7 DNA by Escherichia coli RNA polymerase.大肠杆菌RNA聚合酶对组蛋白覆盖的T7 DNA的转录
Biochemistry. 1978 Dec 26;17(26):5695-705. doi: 10.1021/bi00619a015.
10
Histone modifications in the yeast S. Cerevisiae.酿酒酵母中的组蛋白修饰。
Nucleic Acids Res. 1981 Jul 10;9(13):3205-16. doi: 10.1093/nar/9.13.3205.
引用本文的文献
1
BRD4: an effective target for organ fibrosis.BRD4：器官纤维化的有效靶点。
Biomark Res. 2024 Aug 30;12(1):92. doi: 10.1186/s40364-024-00641-6.
2
Molecular Dynamics Simulations Combined with Markov Model to Explore the Effect of Allosteric Inhibitor Binding on Bromodomain-Containing Protein 4.分子动力学模拟结合马尔可夫模型探索变构抑制剂结合对包含溴结构域蛋白 4 的影响。
Int J Mol Sci. 2023 Jun 29;24(13):10831. doi: 10.3390/ijms241310831.
3
Targeting bromodomain-containing proteins: research advances of drug discovery.靶向含溴结构域蛋白：药物研发的研究进展
Mol Biomed. 2023 May 5;4(1):13. doi: 10.1186/s43556-023-00127-1.
4
Using PDX animal models to identify and stratify adenoid cystic carcinoma patients presenting an enhanced response to HDAC inhibitors.利用人源肿瘤异种移植（PDX）动物模型来识别和分层对组蛋白去乙酰化酶（HDAC）抑制剂反应增强的腺样囊性癌患者。
Am J Cancer Res. 2023 Jan 15;13(1):143-160. eCollection 2023.
5
Bromodomain and BET family proteins as epigenetic targets in cancer therapy: their degradation, present drugs, and possible PROTACs.作为癌症治疗中表观遗传靶点的溴结构域和BET家族蛋白：它们的降解、现有药物及可能的PROTACs。
RSC Adv. 2020 Dec 24;11(2):612-636. doi: 10.1039/d0ra07971e.
6
Histone Deacetylase Inhibitors as Therapeutic Interventions on Cervical Cancer Induced by Human Papillomavirus.组蛋白去乙酰化酶抑制剂作为对人乳头瘤病毒诱导的宫颈癌的治疗干预措施
Front Cell Dev Biol. 2021 Jan 28;8:592868. doi: 10.3389/fcell.2020.592868. eCollection 2020.
7
Prediction of Alzheimer's disease-specific phospholipase c gamma-1 SNV by deep learning-based approach for high-throughput screening.基于深度学习的高通量筛选阿尔茨海默病特异性磷酯酶 Cγ-1 SNV 的预测。
Proc Natl Acad Sci U S A. 2021 Jan 19;118(3). doi: 10.1073/pnas.2011250118.
8
Identification of histone acetylation in a murine model of allergic asthma by proteomic analysis.通过蛋白质组学分析鉴定过敏性哮喘小鼠模型中的组蛋白乙酰化。
Exp Biol Med (Maywood). 2021 Apr;246(8):929-939. doi: 10.1177/1535370220980345. Epub 2020 Dec 16.
9
Brd/BET Proteins Influence the Genome-Wide Localization of the Kaposi's Sarcoma-Associated Herpesvirus and Murine Gammaherpesvirus Major Latency Proteins.溴结构域/溴端外结构域蛋白影响卡波西肉瘤相关疱疹病毒和小鼠γ疱疹病毒主要潜伏蛋白在全基因组范围内的定位。
Front Microbiol. 2020 Oct 22;11:591778. doi: 10.3389/fmicb.2020.591778. eCollection 2020.
10
High-Throughput Quantitative Top-Down Proteomics: Histone H4.高通量定量从头蛋白质组学：组蛋白 H4。
J Am Soc Mass Spectrom. 2019 Dec;30(12):2548-2560. doi: 10.1007/s13361-019-02350-z. Epub 2019 Nov 18.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.组蛋白的乙酰化和甲基化及其在RNA合成调控中的可能作用。
Proc Natl Acad Sci U S A. 1964 May;51(5):786-94. doi: 10.1073/pnas.51.5.786.
3
Histone, a suppressor of chromosomal RNA synthesis.组蛋白，一种染色体RNA合成的抑制剂。
Proc Natl Acad Sci U S A. 1962 Jul 15;48(7):1216-22. doi: 10.1073/pnas.48.7.1216.
4
Template properties of liver chromatin.肝脏染色质的模板特性
J Mol Biol. 1966 Jan;15(1):160-74. doi: 10.1016/s0022-2836(66)80218-8.
5
6
Chemical studies of histone acetylation. The distribution of epsilon-N-acetyllysine in calf thymus histones.组蛋白乙酰化的化学研究。小牛胸腺组蛋白中ε-N-乙酰赖氨酸的分布。
J Biol Chem. 1968 Dec 25;243(24):6361-6.
7
Chemical studies of histone acetylation. The occurrence of epsilon-N-acetyllysine in the f2a1 histone.组蛋白乙酰化的化学研究。f2a1组蛋白中ε-N-乙酰赖氨酸的存在。
J Biol Chem. 1968 Oct 10;243(19):5018-22.
8
Sites of in vivo acetylation in trout testis histone IV.虹鳟鱼睾丸组蛋白IV的体内乙酰化位点
J Biol Chem. 1971 May 25;246(10):3182-8.
9
Fractionation of liver chromatin.肝脏染色质的分级分离
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2941-4. doi: 10.1073/pnas.68.12.2941.
10
Trout testis cells. 3. Acetylation of histones in different cell types from developing trout testis.虹鳟鱼睾丸细胞。3. 发育中的虹鳟鱼睾丸不同细胞类型中组蛋白的乙酰化。
J Biol Chem. 1972 Sep 10;247(17):5506-10.
Zotero 插件