霍乱毒素经烟酰胺腺嘌呤二核苷酸（NAD+）进行光标记。
Photolabelling of cholera toxin by NAD+.
作者信息
Galloway T S, Tait R M, van Heyningen S
出版信息
Biochem J. 1987 Mar 15;242(3):927-30. doi: 10.1042/bj2420927.
When cholera toxin is incubated under u.v. light with NAD+ labelled in either the adenine or the nicotinamide moiety, radioactivity becomes covalently bound to the protein. The reaction is specific for cholera toxin, and is inhibited by excess unlabelled NAD+ or NAD analogues. Only the active A 1 chain of the toxin is labelled. The u.v.-absorption spectrum of the product is very similar to that of NAD+, and shows the same reaction with cyanide. The nature of the product is therefore different from that found when diphtheria toxin is photolabelled [Carroll & Collier (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 3307-3311] in that the yield is lower, but both moieties of the NAD molecule become bound.
当霍乱毒素在紫外线照射下与腺嘌呤或烟酰胺部分标记的NAD⁺一起温育时，放射性会与蛋白质共价结合。该反应对霍乱毒素具有特异性，并被过量的未标记NAD⁺或NAD类似物抑制。只有毒素的活性A1链被标记。产物的紫外吸收光谱与NAD⁺的非常相似，并且与氰化物显示相同的反应。因此，产物的性质与白喉毒素进行光标记时所发现的不同[卡罗尔和科利尔（1984年）《美国国家科学院院刊》81，3307 - 3311]，因为产率较低，但NAD分子的两个部分都会结合。
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