嘌呤霉素和N-（乙基-2-重氮丙二酰基）嘌呤霉素光掺入大肠杆菌核糖体。
Photoincorporation of puromycin and N-(ethyl-2-diazomalonyl)puromycin into Escherichia coli ribosomes.
作者信息
Cooperman B S, Jaynes E N, Brunswick D J, Luddy M A
出版信息
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2974-8. doi: 10.1073/pnas.72.8.2974.
Abstract
[3H]Puromycin and N-(ethyl-2-diazomalonyl)[3H]puromycin are incorporated into E. coli ribosomes on irradiation at 253.7 nm. Both compounds incorporate into both protein and nucleic acid. Two-dimensional gel electrophoresis of ribosomal protein shows that L23 is the major protein labeled by puromycin. Although incorporation is clearly a complex process, evidence is presented that L23 is labeled via an affinity labeling process, thus placing L23 at the aminoacyl-tRNA receptor (A) site. N-(ethyl-2-diazomalonyl)puromycin is a ribosomal ligand, as shown by its inhibition of two ribosomal assays, but it is not a good puromycin analog, and it is unclear whether its incorporation, which proceeds via both carbene-dependent and carbene-independent processes, results from affinity labeling.
摘要
[3H]嘌呤霉素和N-(乙基-2-重氮丙二酰基)[3H]嘌呤霉素在253.7nm光照下可掺入大肠杆菌核糖体。这两种化合物均可掺入蛋白质和核酸中。核糖体蛋白的二维凝胶电泳显示，L23是被嘌呤霉素标记的主要蛋白质。尽管掺入显然是一个复杂的过程，但有证据表明L23是通过亲和标记过程被标记的，因此L23位于氨酰-tRNA受体（A）位点。N-(乙基-2-重氮丙二酰基)嘌呤霉素是一种核糖体配体，这可通过其对两种核糖体检测的抑制作用得到证明，但它不是一种良好的嘌呤霉素类似物，并且尚不清楚其通过卡宾依赖性和卡宾非依赖性过程进行的掺入是否源于亲和标记。
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