Mater Research, Translational Research Institute, Brisbane, Queensland, Australia; School of Public Health, Xi'an Jiaotong University, Xi'an, Shaanxi, PR China.
Mater Research, Translational Research Institute, Brisbane, Queensland, Australia.
Mol Cell Endocrinol. 2022 Oct 1;556:111740. doi: 10.1016/j.mce.2022.111740. Epub 2022 Aug 4.
Dysregulation of adipose tissue involves increased cellular hypoxia, ER stress, and inflammation and altered adipokine production, contributing to the aetiology of obesity-related diseases including type 2 diabetes and cardiovascular disease. This study aimed to investigate the effects of Vitamin C supplementation on these processes in primary human preadipocytes and adipocytes. Treatment of preadipocytes and adipocytes with the proinflammatory cytokine TNFα and palmitic acid (PA), to mimic the obesogenic milieu, significantly increased markers of hypoxia, ER stress and inflammation and reduced secretion of high molecular weight (HMW) adiponectin. Importantly, Vitamin C abolished TNFα+PA induced hypoxia and significantly reduced the increases in ER stress and inflammation in both cell types. Vitamin C also significantly increased the secretion of HMW adiponectin from adipocytes. These findings indicate that Vitamin C can reduce obesity-associated cellular stress and thus provide a rationale for future investigations.
脂肪组织失调涉及细胞缺氧增加、内质网应激和炎症以及改变脂联素的产生，导致肥胖相关疾病（包括 2 型糖尿病和心血管疾病）的发病机制。本研究旨在探讨维生素 C 补充对原代人前体脂肪细胞和脂肪细胞中这些过程的影响。用促炎细胞因子 TNFα 和棕榈酸（PA）处理前体脂肪细胞和脂肪细胞，模拟肥胖环境，显著增加了缺氧、内质网应激和炎症的标志物，并减少了高分子量（HMW）脂联素的分泌。重要的是，维生素 C 消除了 TNFα+PA 诱导的缺氧，并显著降低了两种细胞类型中内质网应激和炎症的增加。维生素 C 还显著增加了脂肪细胞中 HMW 脂联素的分泌。这些发现表明，维生素 C 可以减少与肥胖相关的细胞应激，从而为未来的研究提供了依据。
