State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, China.
Shandong Huishilai Biotechnology Co., Ltd, Jinan, China.
Adv Appl Microbiol. 2022;119:35-81. doi: 10.1016/bs.aambs.2022.05.002. Epub 2022 Jun 21.
Bio-based plastics production offers an alternative to the environmental problems posed by a significant reliance on fossil fuels. While dicarboxylic acids were essential bioplastic monomers, producing them on a large scale proved problematic. Recently, metabolic engineering has opened up interesting possibilities for producing dicarboxylic acids sustainably and efficiently. In this chapter, studies on the development of several dicarboxylic acid bioplastic monomers were presented. Furthermore, for different dicarboxylic acids, a variety of metabolic engineering strategies were highlighted, including improving the utilization rate of substrates, strengthening the catalytic efficiency of key enzymes, blocking branching pathways to balance metabolic flux, and improving cell physiological performance to promote biosynthesis. Finally, the remaining obstacles and solutions for building advanced dicarboxylic acid microbial systems were discussed.
生物基塑料的生产为解决对化石燃料的严重依赖所带来的环境问题提供了一种替代方案。虽然二羧酸是重要的生物塑料单体，但大规模生产这些单体存在问题。最近，代谢工程为可持续、高效地生产二羧酸开辟了有趣的可能性。本章介绍了几种二羧酸生物塑料单体的开发研究。此外，针对不同的二羧酸，还强调了各种代谢工程策略，包括提高底物利用率、增强关键酶的催化效率、阻断分支途径以平衡代谢流，以及改善细胞生理性能以促进生物合成。最后，讨论了构建先进的二羧酸微生物系统所面临的剩余障碍和解决方案。
