Wu Xinyi, Cui Le, Wang Haoquan, Xu Jinhong, Zhong Zhaozhao, Jia Xibei, Wang Jiaqi, Zhang Huahua, Shi Yanteng, Tang Yuhang, Yang Qianhui, Liang Qiongdan, Zhang Yujing, Li Jing, Jiang Xiaohong
State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute for Life Sciences (NAILS), Nanjing University, 163 Xianlin Road, Nanjing, 210046, Jiangsu, China.
Eur J Nutr. 2023 Feb;62(1):199-211. doi: 10.1007/s00394-022-02980-2. Epub 2022 Aug 7.
Overconsumption of sugar-sweetened beverages (SSBs) is associated with an increased risk of metabolic disorders, including obesity and diabetes. However, accumulating evidence also suggests the potential negative impact of consuming nonnutritive sweeteners (NNSs) on weight and glycaemic control. The metabolic effects of sucralose, the most widely used NNS, remain controversial. This study aimed to compare the impact of intake of dietary sucralose (acceptable daily intake dose, ADI dose) and sucrose-sweetened water (at the same sweetness level) on lipid and glucose metabolism in male mice.
Sucralose (0.1 mg/mL) or sucrose (60 mg/mL) was added to the drinking water of 8-week-old male C57BL/6 mice for 16 weeks, followed by oral glucose and intraperitoneal insulin tolerance tests, and measurements of bone mineral density, plasma lipids, and hormones. After the mice were sacrificed, the duodenum and ileum were used for examination of sweet taste receptors (STRs) and glucose transporters.
A significant increase in fat mass was observed in the sucrose group of mice after 16 weeks of sweetened water drinking. Sucrose consumption also led to increased levels of plasma LDL, insulin, lipid deposition in the liver, and increased glucose intolerance in mice. Compared with the sucrose group, mice consuming sucralose showed much lower fat accumulation, hyperlipidaemia, liver steatosis, and glucose intolerance. In addition, the daily dose of sucralose only had a moderate effect on T1R2/3 in the intestine, without affecting glucose transporters and plasma insulin levels.
Compared with mice consuming sucrose-sweetened water, daily drinking of sucralose within the ADI dose had a much lower impact on glucose and lipid homeostasis.
饮用含糖饮料（SSB）过量与包括肥胖和糖尿病在内的代谢紊乱风险增加有关。然而，越来越多的证据也表明食用非营养性甜味剂（NNS）对体重和血糖控制可能产生负面影响。最广泛使用的非营养性甜味剂三氯蔗糖的代谢效应仍存在争议。本研究旨在比较雄性小鼠摄入膳食三氯蔗糖（可接受的每日摄入量，ADI剂量）和蔗糖水（相同甜度水平）对脂质和葡萄糖代谢的影响。
将三氯蔗糖（0.1毫克/毫升）或蔗糖（60毫克/毫升）添加到8周龄雄性C57BL/6小鼠的饮用水中，持续16周，随后进行口服葡萄糖和腹腔内胰岛素耐量试验，并测量骨密度、血脂和激素水平。小鼠处死后，取十二指肠和回肠检测甜味受体（STR）和葡萄糖转运蛋白。
饮用蔗糖水16周后，蔗糖组小鼠的脂肪量显著增加。摄入蔗糖还导致小鼠血浆低密度脂蛋白、胰岛素水平升高，肝脏脂质沉积增加，葡萄糖耐量降低。与蔗糖组相比，摄入三氯蔗糖的小鼠脂肪堆积、高脂血症、肝脏脂肪变性和葡萄糖耐量降低的程度要低得多。此外，三氯蔗糖的每日剂量对肠道中的T1R2/3只有中等程度的影响，不影响葡萄糖转运蛋白和血浆胰岛素水平。
与饮用蔗糖水的小鼠相比，在ADI剂量内每日饮用三氯蔗糖对葡萄糖和脂质稳态的影响要低得多。
