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适体二聚化启发的仿生夹心法用于基于阻抗的新型冠状病毒抗原检测。

Aptamers dimerization inspired biomimetic clamp assay towards impedimetric SARS-CoV-2 antigen detection.

作者信息

Han Cong, Xing Wenping, Li Wenjin, Fang Xiaona, Zhao Jian, Ge Feng, Ding Wei, Qu Pengpeng, Luo Zhaofeng, Zhang Liyun

机构信息

State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300350, China.

State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300350, China.

出版信息

Sens Actuators B Chem. 2023 Apr 1;380:133387. doi: 10.1016/j.snb.2023.133387. Epub 2023 Jan 20.

DOI:10.1016/j.snb.2023.133387
PMID:36694572
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9851723/
Abstract

Antigen-detecting rapid diagnostic testing (Ag-RDT) has contributed to containing the spread of SARS-CoV-2 variants of concern (VOCs). In this study, we proposed a biomimetic clamp assay for impedimetric SARS-CoV-2 nucleocapsid protein (Np) detection. The DNA biomimetic clamp (DNA-BC) is formed by a pair of Np aptamers connected via a T spacer. The 5'- terminal of the DNA-BC is phosphate-modified and then anchored on the surface of the screen-printed gold electrode, which has been pre-coated with Au@UiO-66-NH. The integrated DNA-material sensing biochip is fabricated through the strong Zr-O-P bonds to form a clamp-type impedimetric aptasensor. It is demonstrated that the aptasensor could achieve Np detection in one step within 11 min and shows pronounced sensitivity with a detection limit of 0.31 pg mL. Above all, the aptasensor displays great specificity and stability under physiological conditions as well as various water environments. It is a potentially promising strategy to exploit reliable Ag-RDT products to confront the ongoing epidemic.

摘要

抗原检测快速诊断测试（Ag-RDT）有助于遏制严重急性呼吸综合征冠状病毒2（SARS-CoV-2）变异株（VOCs）的传播。在本研究中，我们提出了一种用于阻抗式检测SARS-CoV-2核衣壳蛋白（Np）的仿生钳夹分析方法。DNA仿生钳夹（DNA-BC）由一对通过T间隔区连接的Np适配体形成。DNA-BC的5'-末端经过磷酸化修饰，然后固定在预先涂覆有Au@UiO-66-NH的丝网印刷金电极表面。通过强Zr-O-P键形成钳夹型阻抗适配体传感器，制备出集成DNA-材料传感生物芯片。结果表明，该适配体传感器能够在11分钟内一步完成Np检测，具有显著的灵敏度，检测限为0.31 pg/mL。最重要的是，该适配体传感器在生理条件以及各种水环境下均表现出高度的特异性和稳定性。开发可靠的Ag-RDT产品是应对当前疫情的一种潜在的有前景的策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/d9dcadaa5f6e/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/3270bf096330/sc1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/22fbc4e624bf/sc2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/6434d1b2eac1/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/435d2c4f81f4/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/871ad18acd4b/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/d9dcadaa5f6e/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/3270bf096330/sc1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/22fbc4e624bf/sc2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/6434d1b2eac1/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/435d2c4f81f4/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/871ad18acd4b/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0589/9851723/d9dcadaa5f6e/gr4_lrg.jpg

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