College of Chemistry and Environmental Engineering, Changchun University of Science and Technology, Changchun 130022, PR China.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Dec 5;282:121683. doi: 10.1016/j.saa.2022.121683. Epub 2022 Jul 26.
Metal nitrogen-doped carbon (MNC) nanozymes have received increasing attention in bio-catalysis filed due to adequate catalytic activity, outstanding stability and reusability. Herein, the Fe/NC nanozymes (Fe/NC NZs) with peroxidase-like activity was successfully synthesized and a fluorescence turn on and colorimetric dual-mode sensing system was developed for quantification of captopril (CP) based on Fe/NC NZs and orange-emitting carbon dots (O-CDs). The Fe/NC NZs as an enzyme mimic can efficiently catalyze the 3,3',5,5'-tetramethylbenzidine (TMB) chromogenic reaction, forming blue-colored oxidized TMB product (oxTMB) with the presence of HO, leading to the fluorescence quenching of O-CDs simultaneously via the inner filter effect (IFE). When CP was present, the blue oxTMB was reduced to colorless TMB, resulting in the inhibition of IFE and the recovery of fluorescence of O-CDs. The fluorescence increase of O-CDs and absorbance decrease of oxTMB depended on CP concentration. Good linear relationships of fluorescence and colorimetric sensing towards CP were obtained in the range from 1 to 50 μM, and the detection limits were 0.47 and 0.56 μM, respectively. Moreover, this as-constructed dual-mode sensor was used to detect CP in pharmaceutical products with satisfactory results.
金属氮掺杂碳 (MNC) 纳米酶由于具有足够的催化活性、出色的稳定性和可重复使用性，在生物催化领域受到越来越多的关注。本文成功合成了具有过氧化物酶样活性的 Fe/NC 纳米酶 (Fe/NC NZs)，并基于 Fe/NC NZs 和橙色发光碳点 (O-CDs) 开发了用于检测卡托普利 (CP) 的荧光开启和比色双模式传感系统。作为酶模拟物的 Fe/NC NZs 可以有效地催化 3,3',5,5'-四甲基联苯胺 (TMB) 显色反应，在 HO 的存在下形成蓝色氧化 TMB 产物 (oxTMB)，同时通过内滤效应 (IFE) 使 O-CDs 的荧光猝灭。当 CP 存在时，蓝色 oxTMB 被还原为无色 TMB，导致 IFE 抑制和 O-CDs 荧光恢复。O-CDs 的荧光增强和 oxTMB 的吸光度降低取决于 CP 的浓度。在 1 至 50 μM 的范围内，对 CP 的荧光和比色传感均获得了良好的线性关系，检测限分别为 0.47 和 0.56 μM。此外，该构建的双模式传感器用于检测药物制剂中的 CP，结果令人满意。
