Lv Yuntai, Zhou Chenyu, Li Meini, Huo Zejiao, Wei Zhiyuan, Wang Nan, Wang Guannan, Su Xingguang
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
School of Pharmacy, Shenyang Medical University, Shenyang,110034, China.
Talanta. 2024 Feb 1;268(Pt 1):125328. doi: 10.1016/j.talanta.2023.125328. Epub 2023 Oct 20.
In this research, a sensitive fluorometric and colorimetric dual-mode sensing platform based on nitrogen-doped carbon quantum dots (NCDs) and magnetic Fe nanoparticles with peroxidase-like activity (Fe nanozymes, Fe NZs) was established, and was further applied for the detection of α-glucosidase (α-glu) and its inhibitors. The ⋅OH that produced by HO catalyzed by Fe NZs can oxidize the colorless diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) to green oxABTS, and a noticeable absorption peak at 417 nm appeared. Simultaneously, oxABTS can quench the fluorescence of NCDs at 402 nm via fluorescence resonance energy transfer (FRET). 2-O-α-D-glucopyranosyl-L-ascorbic acid (AAG) can be decomposed by α-glu to glucose and ascorbic acid (AA), AA can prevent the oxidation of ABTS, resulting in the absorption at 417 nm decreased. Moreover, the quenching effect of oxABTS on NCDs is weakened, and the fluorescence at 402 nm is restored. Therefore, based on the change of absorption at 417 nm and fluorescence at 402 nm, the fluorometric and colorimetric dual-mode sensing method can be used for the determination of acarbose and voglibose that are the inhibitors of α-glu.
本研究构建了一种基于氮掺杂碳量子点（NCDs）和具有过氧化物酶样活性的磁性铁纳米颗粒（铁纳米酶，Fe NZs）的灵敏荧光和比色双模式传感平台，并将其进一步应用于α-葡萄糖苷酶（α-glu）及其抑制剂的检测。由Fe NZs催化H₂O₂产生的·OH可将无色的2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）氧化为绿色的氧化型ABTS（oxABTS），在417 nm处出现明显的吸收峰。同时，oxABTS可通过荧光共振能量转移（FRET）淬灭402 nm处NCDs的荧光。2-O-α-D-吡喃葡萄糖基-L-抗坏血酸（AAG）可被α-glu分解为葡萄糖和抗坏血酸（AA），AA可阻止ABTS的氧化，导致417 nm处的吸收降低。此外，oxABTS对NCDs的淬灭作用减弱，402 nm处的荧光恢复。因此，基于417 nm处吸收和402 nm处荧光的变化，该荧光和比色双模式传感方法可用于测定作为α-glu抑制剂的阿卡波糖和伏格列波糖。
