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用于水果总抗氧化能力测定的铜掺杂和2-丙基咪唑修饰的纳米氧化铈（CeO@Cu-PrIm）类氧化酶纳米酶

Cu-doped and 2-propylimidazole-modified nanoceria (CeO@Cu-PrIm) oxidase-like nanozyme for total antioxidant capacity assay of fruits.

作者信息

Fu Zhendong, Qiu Jiahe, Gong Ping, Zhang Danhong, Wang Liping

机构信息

Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University Changchun 130012 China

Jilin University Hospital, Jilin University Changchun 130012 China

出版信息

RSC Adv. 2025 Apr 1;15(13):9997-10004. doi: 10.1039/d4ra07858f. eCollection 2025 Mar 28.

DOI:10.1039/d4ra07858f
PMID:40171292
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11959357/
Abstract

The precise and sensitive quantification of total antioxidant capacity (TAC) is indispensable for evaluating the quality of foods rich in antioxidants. In this investigation, a novel nanozyme exhibiting robust oxidase activity was synthesized a sonochemical doping process utilizing copper ions and the modification of 2-propylimidazole onto nanoceria. Subsequently, a CeO2@Cu-PrIm/ox-TMB assay system was successfully formulated, furnishing a practical and highly sensitive analytical tool for TAC determination. The colorimetric sensor exhibited a linear response over a concentration range of 1 μM to 70 μM, accompanied by a limit of detection (LOD) of 1.26 μM under meticulously screened conditions. This assay displayed substantial practical utility for TAC analysis in fruits, attributed to its exceptional accuracy and selectivity. This research endeavor may offer a novel direction for the design of nanozymes and colorimetric biosensors possessing heightened oxidase activity, thereby advancing the field of analytical chemistry and food science.

摘要

准确而灵敏地定量总抗氧化能力（TAC）对于评估富含抗氧化剂的食品质量而言不可或缺。在本研究中，通过利用铜离子的声化学掺杂过程以及在纳米氧化铈上修饰2-丙基咪唑，合成了一种具有强大氧化酶活性的新型纳米酶。随后，成功构建了CeO2@Cu-PrIm/ox-TMB检测系统，为TAC测定提供了一种实用且高度灵敏的分析工具。该比色传感器在1 μM至70 μM的浓度范围内呈现线性响应，在精心筛选的条件下检测限（LOD）为1.26 μM。由于其卓越的准确性和选择性，该检测方法在水果TAC分析中具有显著的实际应用价值。本研究工作可能为设计具有更高氧化酶活性的纳米酶和比色生物传感器提供新方向，从而推动分析化学和食品科学领域的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a4a/11959357/52e0de081268/d4ra07858f-f7.jpg
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Synergistic effect of silver nanoclusters and graphene oxide on visible light-driven oxidase-like activity: Construction of a sustainable nanozyme for total antioxidant capacity detection.
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