Nakano M, Otsuka M, Akama K, Tobita T
Biochem Int. 1984 Jul;9(1):39-43.
The isolated zona pellucida from porcine ova was effectively solubilized in water at 60 degrees C within one hour. The circular dichroic spectra of zona in water with and without dithiothreitol showed the beta-form. Although sodium dodecyl sulfate partially induced helical structure, the beta-form was considerably retained. These results indicate that the zona glycoproteins have a structure-forming potential for the beta-structure and the hydrogen bonds of the beta-structure stabilize the supramolecular complex of the zona pellucida. The beta-form was also detected in zona solubilized by tryptic digestion. Porcine acrosin, however, did not solubilize the zona.
从猪卵中分离出的透明带在60摄氏度的水中1小时内即可有效溶解。在添加和不添加二硫苏糖醇的情况下，水中透明带的圆二色光谱显示为β型。尽管十二烷基硫酸钠部分诱导了螺旋结构，但β型仍大量保留。这些结果表明，透明带糖蛋白具有形成β结构的潜力，且β结构的氢键稳定了透明带的超分子复合物。在经胰蛋白酶消化溶解的透明带中也检测到了β型。然而，猪顶体蛋白酶并不能溶解透明带。
