Farid Armita, Sheibani Mohammad, Shojaii Asie, Noori Mitra, Motevalian Manijeh
Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran; Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran.
J Ethnopharmacol. 2022 Nov 15;298:115595. doi: 10.1016/j.jep.2022.115595. Epub 2022 Aug 4.
Plantago major (P. major) has traditionally been used in Iranian Persian medicine to treat gastrointestinal ulcers and bleeding.
This study aimed to investigate the anti-inflammatory effects of the leaf and seed extracts of P. major in rats with acetic acid-induced ulcerative colitis (UC).
To this end, 49 rats were randomly divided into seven groups. UC was induced in all groups but the control (vehicle) group using a single intra-rectal administration of 2 ml of 4% acetic acid. Other groups received daily intraperitoneal (i.p.) injections of the seed extract of P. major (400 mg/kg and 700 mg/kg), the leaf extract of P. major (400 mg/kg and 700 mg/kg), and sulfasalazine (400 mg/kg) for seven consecutive days, respectively. The rats' rectum was surgically removed and evaluated for macroscopic and microscopic damage. The tissue levels of oxidative stress and inflammatory markers were measured using the ELISA method.
The high-dose leaf extract significantly decreased ulcer index and histopathologic damage as well as the tissue levels of IL-6, TNF-α, PGE2, IL-1β, MPO, and MDA compared to the damage group. The low-dose leaf extract also significantly reduced the levels of some markers. The seed extract in the two used doses caused a modest decrease in the histopathological damages and ulcer index.
P. major leaf extract effectively reduces inflammation and mucosal damage in rats with UC, especially when administered in high doses. P. major seed extract has minimal protective effects on UC.
大车前草在伊朗传统波斯医学中一直被用于治疗胃肠道溃疡和出血。
本研究旨在探讨大车前草叶和种子提取物对乙酸诱导的溃疡性结肠炎（UC）大鼠的抗炎作用。
为此，将49只大鼠随机分为7组。除对照组（赋形剂）外，其余各组均通过单次直肠内注射2 ml 4%乙酸诱导UC。其他组分别连续7天每日腹腔注射大车前草种子提取物（400 mg/kg和700 mg/kg）、大车前草叶提取物（400 mg/kg和700 mg/kg）和柳氮磺胺吡啶（400 mg/kg）。手术切除大鼠直肠并评估宏观和微观损伤。采用ELISA法测定氧化应激和炎症标志物的组织水平。
与损伤组相比，高剂量叶提取物显著降低溃疡指数、组织病理学损伤以及IL-6、TNF-α、PGE2、IL-1β、MPO和MDA的组织水平。低剂量叶提取物也显著降低了一些标志物的水平。两种剂量的种子提取物均使组织病理学损伤和溃疡指数略有降低。
大车前草叶提取物能有效减轻UC大鼠的炎症和黏膜损伤，尤其是高剂量给药时。大车前草种子提取物对UC的保护作用最小。
