Zhao Hongliang, Gao Hongxiang, Kang Jing, Chen Jiexin, Ouyang Huiling, Chen Peiyao, Althaf Hussain Shaik, Alrubie Turki Mayudh, Narendra Maddu, Wang Kai
Department of Hematology & Oncology, Honghui Hospital, Xi An Jiao Tong University, Xian, 710054, People's Republic of China.
Department of Zoology, College of Science, King Saud University, 11451, Riyadh, Saudi Arabia.
Discov Oncol. 2025 May 14;16(1):764. doi: 10.1007/s12672-025-02436-z.
We aimed to evaluate the potential effects of Plantago major (PM) to enhance the radiosensitivity of human breast adenocarcinoma cell line (MCF-7) in an animal model.
Seventy-two female Balb/c mice were divided into nine groups (8 mice per group) as follows: MCF-7 breast cancer control group, MCF-7+low dose of PM (1000 mg/kg), MCF-7+high dose of PM (1500 mg/kg), MCF-7+3 Gy superficial X-ray, MCF-7+5 Gy superficial X-ray, MCF-7+1000&1500 mg/kg of PM with 3 and 5 Gy irradiations. For each treatment group, micronuclei in 500 binucleate MCF-7 cells from a minimum of three experiments were counted. The alkaline comet assay was used to calculate % DNA in the tail and % of apoptotic comets. The tumor size in the treated groups (3 mice per group) was assessed at 4- and 8-weeks post-treatment.
The number of total micronuclei and binucleated micronuclei in the PM and/or irradiation treated groups was significantly higher than in the control group (P < 0.05). Irradiation+PM resulted in a significant increase treatment effect compared to the irradiation-only groups (P < 0.01). In addition, the higher PM concentration had a significantly higher number of micronucleated binucleate cells when combined with 5 Gy irradiation (P = 0.022). Irradiation alone or in combination with PM resulted in significant increases in percentages of DNA in tail and apoptotic comet values compared to the PM-only treatment groups (P < 0.02). Combining 5 Gy of irradiation with 1000 mg/kg of PM led to a 26% reduction in tumor size (0.28±0.04 vs. 0.38 ± 0.03) after 4 weeks, and combining 5 Gy of irradiation with 1500 mg/kg of PM resulted in a 40% decrease in tumor volume after 4 weeks (0.21±0.04 vs. 0.35±0.03).
PM extract at both doses demonstrated an antitumor effect on induced MCF-7 breast cancer tumors, with this effect being enhanced when combined with irradiation.
我们旨在评估大车前草（PM）在动物模型中增强人乳腺癌细胞系（MCF - 7）放射敏感性的潜在作用。
72只雌性Balb/c小鼠分为9组（每组8只），如下：MCF - 7乳腺癌对照组、MCF - 7 +低剂量PM（1000毫克/千克）、MCF - 7 +高剂量PM（1500毫克/千克）、MCF - 7 + 3戈瑞浅表X射线、MCF - 7 + 5戈瑞浅表X射线、MCF - 7 + 1000及1500毫克/千克的PM并分别接受3戈瑞和5戈瑞照射。对于每个治疗组，从至少三个实验中计数500个双核MCF - 7细胞中的微核。采用碱性彗星试验计算尾部DNA百分比和凋亡彗星百分比。在治疗后4周和8周评估治疗组（每组3只小鼠）的肿瘤大小。
PM和/或照射治疗组的总微核和双核微核数量显著高于对照组（P < 0.05）。与仅照射组相比，照射 + PM导致治疗效果显著增加（P < 0.01）。此外，当与5戈瑞照射联合时，较高浓度的PM具有显著更多的微核双核细胞（P = 0.022）。与仅PM治疗组相比，单独照射或照射与PM联合导致尾部DNA百分比和凋亡彗星值显著增加（P < 0.02）。4周后，5戈瑞照射与1000毫克/千克的PM联合导致肿瘤大小减少26%（0.28±0.04对0.38±0.03），5戈瑞照射与1500毫克/千克的PM联合在4周后导致肿瘤体积减少40%（0.21±0.04对0.35±0.03）。
两种剂量的PM提取物对诱导的MCF - 7乳腺癌肿瘤均显示出抗肿瘤作用，与照射联合时该作用增强。
