Mok Tsz Ngai, He Qiyu, Zhang Xiaoxi, Sin Tat Hang, Wang Huajun, Hou Huige, Pan Jinghua, Zheng Xiaofei, Zha Zhengang, Li Jieruo
Department of Orthopedic Surgery and Sports Medicine Center, The First Affiliated Hospital and The First Clinical College, Jinan University, Guangzhou, China.
Pediatric Cardiac Surgery Center, National Center for Cardiovascular Disease and Fuwai Hospital, Chinese Academy of Medical Sciences, Peking Union Medical, Beijing, China.
Front Pharmacol. 2022 Jul 22;13:919104. doi: 10.3389/fphar.2022.919104. eCollection 2022.
Tendon impairment is a common injury associated with impairment of range of motion and pain. Currently, evidence has confirmed that natural herbs contribute to orthopedics and have shown excellent results in the clinical management of tendon impairment. Shujin Huoxue tablet (SHT) and its complex prescriptions are regularly used in tendon rupture therapy with positive results. This study aimed to discover the potential molecules that promote tendon healing. The Chinese traditional medicine system pharmacological database analysis platform (TCMSP) is the primary resource. The Traditional Chinese Medicine Integrated Database and Encyclopedia of Traditional Chinese Medicine database were used as secondary databases. The GeneCards database was used to search for reported tendinopathy-related genes by keywords. Functions of the targeted genes were analyzed using Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes. Protein-protein interaction information was extracted from the STRING database. Docking study, MTT assay, quantitative real-time PCR, and migration assays were performed to obtain a better understanding of the herbs according to cell function to test the basic pharmacological action . A total of 104 disease nodes, 496 target gene nodes, 35 ingredient nodes, and one drug node were extracted. According to the TCMSP database, 6-hydroxykaempferol, which reportedly promotes the proliferation of microvascular endothelial cells, is a molecule found in SHT. We found that it promoted the proliferation and migration of tendon fibroblasts and elevated tendon repair-related gene expression. Purified 6-hydroxykaempferol promoted the proliferation and migration of tendon fibroblasts and increased their mRNA expression in tendon proliferation.
肌腱损伤是一种常见的损伤，常伴有活动范围受限和疼痛。目前，已有证据证实天然草药对骨科有益，并在肌腱损伤的临床治疗中显示出优异的效果。舒筋活血片（SHT）及其复方制剂常用于肌腱断裂治疗，效果良好。本研究旨在发现促进肌腱愈合的潜在分子。以中药系统药理学数据库分析平台（TCMSP）作为主要资源，使用中医综合数据库和中医百科全书数据库作为辅助数据库。通过关键词在GeneCards数据库中搜索已报道的肌腱病相关基因。利用基因本体富集分析和京都基因与基因组百科全书对靶向基因的功能进行分析。从STRING数据库中提取蛋白质-蛋白质相互作用信息。进行对接研究、MTT实验、定量实时PCR和迁移实验，以便根据细胞功能更好地了解草药，测试其基本药理作用。共提取出104个疾病节点、496个靶基因节点、35个成分节点和1个药物节点。根据TCMSP数据库，据报道可促进微血管内皮细胞增殖的6-羟基山奈酚是在舒筋活血片中发现的一种分子。我们发现它促进了肌腱成纤维细胞的增殖和迁移，并提高了肌腱修复相关基因的表达。纯化的6-羟基山奈酚促进了肌腱成纤维细胞的增殖和迁移，并增加了它们在肌腱增殖中的mRNA表达。
