Du Xiaolong, He Xiaoyun, Liu Qingqing, Liu Qiuyue, Di Ran, Chu Mingxing
Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing, China.
College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Front Vet Sci. 2022 Jul 22;9:888207. doi: 10.3389/fvets.2022.888207. eCollection 2022.
In seasonal estrus, it is well known that melatonin-regulated biorhythm plays a key role. Some studies indicate that the adrenal gland plays an important role in reproduction in mammals, but the molecular mechanism is not clear. This study used an artificially controlled light photoperiod model, combined with RNA-seq technology and bioinformatics analysis, to analyze the messenger RNA (mRNA) and microRNA (miRNA) of ewe (Sunite) adrenal glands under different photoperiod treatments. After identification, the key candidate genes and that photoperiod affects reproduction were confirmed. The miRNAs (oar-miR-544-3p, oar-miR-411b-5p, oar-miR-376e-3p, oar-miR-376d, oar-miR-376b-3p, oar-miR-376a-3p) were specifically expressed in the adrenal gland. The candidate mRNA-miRNA pairs (e.g., coagulated by novel miRNA554, novel miRNA555 and novel miRNA559) may affect seasonal estrus. In summary, we constructed relation network of the mRNAs and miRNAs of sheep adrenal glands using RNA sequencing and bioinformatics analysis, thereby, providing a valuable genetic variation resource for sheep genome research, which will contribute to the study of complex traits in sheep.
在季节性发情中，褪黑素调节的生物节律起着关键作用，这是众所周知的。一些研究表明，肾上腺在哺乳动物的繁殖中起重要作用，但其分子机制尚不清楚。本研究采用人工控制光照光周期模型，结合RNA测序技术和生物信息学分析，对不同光周期处理下的苏尼特母羊肾上腺信使核糖核酸（mRNA）和微小核糖核酸（miRNA）进行分析。经鉴定，证实了光周期影响繁殖的关键候选基因。这些miRNA（oar-miR-544-3p、oar-miR-411b-5p、oar-miR-376e-3p、oar-miR-376d、oar-miR-376b-3p、oar-miR-376a-3p）在肾上腺中特异性表达。候选mRNA-miRNA对（如由新型miRNA554、新型miRNA555和新型miRNA559凝结）可能影响季节性发情。总之，我们利用RNA测序和生物信息学分析构建了绵羊肾上腺mRNA和miRNA的关系网络，从而为绵羊基因组研究提供了有价值的遗传变异资源，这将有助于绵羊复杂性状的研究。
