Wang Duoduo, Jin Shunda, Chen Zhe, Shan Yue, Li Lei
College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, China.
School of Integrative Plant Science, Cornell University, Ithaca, NY, United States.
Front Plant Sci. 2022 Jul 22;13:940284. doi: 10.3389/fpls.2022.940284. eCollection 2022.
Pectin methylesterase inhibitors (PMEIs) modulate the status of pectin methylesterification by inhibiting the activity of pectin methylesterase (PME). Recent advances indicate PMEIs play an important role in regulating plant cell wall properties and defense responses. In this study, a genome-wide analysis of gene family in () was conducted and the expression patterns of genes in response to () was investigated. A total of 190 PMEI proteins were identified from the genome of . Chromosomal location, gene structure and properties of the PMEI family were analyzed, and these features were compared with . A total of 123 syntenic ortholog pairs were detected from family by synteny analysis. Results showed the expansion of genes was likely predominately from whole-genome duplication (WGD) or segmental duplications. Multiple -elements related to plant growth and development, environmental stress responses, hormone responses were detected in the promoters of genes, implying they were regulated by both internal and external factors. Furthermore, expression analysis of transcriptome data combined with quantitative RT-PCR (qRT-PCR) validation identified several candidates that were strongly responsive to infection. These genes are candidates for manipulation to breed novel and improved genotypes that are more resistant to sclerotinia stem rot (SSR). Extensive interactions were detected among 30 BnPMEI proteins, forming complex protein-protein interaction networks. Besides, 48 BnPMEIs showed interactions with other proteins including a range of cell wall structure-related enzymes. This study provides new insights into the evolution and function of PMEIs in and lays a foundation for breeding novel genotypes for crop improvement.
果胶甲酯酶抑制剂（PMEIs）通过抑制果胶甲酯酶（PME）的活性来调节果胶甲基酯化状态。最新研究进展表明，PMEIs在调节植物细胞壁特性和防御反应中发挥着重要作用。在本研究中，对油菜（）中的基因家族进行了全基因组分析，并研究了基因在响应核盘菌（）时的表达模式。从油菜基因组中总共鉴定出190个PMEI蛋白。分析了PMEI家族的染色体定位、基因结构和特性，并将这些特征与其他物种进行了比较。通过共线性分析，从该家族中检测到总共123对同线直系同源基因对。结果表明，油菜基因的扩增可能主要源于全基因组复制（WGD）或片段重复。在油菜基因的启动子中检测到多个与植物生长发育、环境胁迫响应、激素响应相关的顺式作用元件，这意味着它们受内部和外部因素的共同调控。此外，结合转录组数据的表达分析和定量RT-PCR（qRT-PCR）验证，鉴定出了几个对核盘菌感染有强烈响应的候选基因。这些油菜基因是培育对菌核病（SSR）更具抗性的新型改良基因型的候选操作对象。在30个BnPMEI蛋白之间检测到广泛的相互作用，形成了复杂的蛋白质-蛋白质相互作用网络。此外，48个BnPMEIs与包括一系列细胞壁结构相关酶在内的其他蛋白质存在相互作用。本研究为油菜中PMEIs的进化和功能提供了新的见解，并为培育用于作物改良的新型基因型奠定了基础。
