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液泡硝酸盐外流需要多种功能冗余的硝酸盐转运蛋白。 （原句结尾的“in.”表述不完整，可能影响准确理解，这里按合理推测翻译）

Vacuolar nitrate efflux requires multiple functional redundant nitrate transporter in .

作者信息

Lu Yu-Ting, Liu De-Fen, Wen Ting-Ting, Fang Zi-Jun, Chen Si-Ying, Li Hui, Gong Ji-Ming

机构信息

National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.

University of Chinese Academy of Sciences, Beijing, China.

出版信息

Front Plant Sci. 2022 Jul 22;13:926809. doi: 10.3389/fpls.2022.926809. eCollection 2022.

DOI:10.3389/fpls.2022.926809
PMID:35937356
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9355642/
Abstract

Nitrate in plants is preferentially stored in vacuoles; however, how vacuolar nitrate is reallocated and to which biological process(es) it might contribute remain largely elusive. In this study, we functionally characterized three nitrate transporters NPF5.10, NPF5.14, and NPF8.5 that are tonoplast-localized. Ectopic expression in oocytes revealed that they mediate low-affinity nitrate transport. Histochemical analysis showed that these transporters were expressed preferentially in pericycle and xylem parenchyma cells. , , and overexpression significantly decreased vacuolar nitrate contents and nitrate accumulation in shoots. Further analysis showed that the sextuple mutant () had a higher NO3-uptake rate than the wild-type Col-0, but no significant difference was observed for nitrate accumulation between them. The septuple mutant () generated by using CRISPR/Cas9 showed essentially decreased nitrate reallocation compared to wild type when exposed to nitrate starvation, though no further decrease was observed when compared to . Notably, , , and as well as , and were consistently induced by mannitol, and more nitrate was detected in the sextuple mutant than in the wild type after mannitol treatment. These observations suggest that vacuolar nitrate efflux is regulated by several functional redundant nitrate transporters, and the reallocation might contribute to osmotic stress response other than mineral nutrition.

摘要

植物中的硝酸盐优先储存在液泡中；然而，液泡中的硝酸盐如何重新分配以及它可能对哪些生物学过程有贡献，在很大程度上仍然不清楚。在本研究中，我们对定位于液泡膜的三个硝酸盐转运蛋白NPF5.10、NPF5.14和NPF8.5进行了功能表征。在卵母细胞中的异位表达表明它们介导低亲和力的硝酸盐转运。组织化学分析表明，这些转运蛋白优先在中柱鞘和木质部薄壁细胞中表达。NPF5.10、NPF5.14和NPF8.5的过表达显著降低了液泡中的硝酸盐含量以及地上部的硝酸盐积累。进一步分析表明，六重突变体（npf5.10 npf5.14 npf8.5）的NO3-吸收速率高于野生型Col-0，但它们之间的硝酸盐积累没有显著差异。使用CRISPR/Cas9产生的七重突变体（npf5.10 npf5.14 npf8.5 nrt1.1 nrt1.2 nrt2.1 nrt2.2）在硝酸盐饥饿条件下与野生型相比，硝酸盐重新分配基本上减少，尽管与六重突变体相比没有进一步减少。值得注意的是，NPF5.10、NPF5.14和NPF8.5以及NRT1.1、NRT1.2和NRT2.1一直被甘露醇诱导，甘露醇处理后六重突变体中检测到的硝酸盐比野生型更多。这些观察结果表明，液泡硝酸盐外流受几个功能冗余的硝酸盐转运蛋白调节，并且这种重新分配可能有助于渗透胁迫响应而非矿质营养。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/20f3e952efde/fpls-13-926809-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/a7ecd5cb3219/fpls-13-926809-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/d1f2427ad3a7/fpls-13-926809-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/795946ef6569/fpls-13-926809-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/ee19ebf799ed/fpls-13-926809-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/c91c83457c69/fpls-13-926809-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/eb5e936ecb78/fpls-13-926809-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/20f3e952efde/fpls-13-926809-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/a7ecd5cb3219/fpls-13-926809-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/d1f2427ad3a7/fpls-13-926809-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/795946ef6569/fpls-13-926809-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/ee19ebf799ed/fpls-13-926809-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/c91c83457c69/fpls-13-926809-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/eb5e936ecb78/fpls-13-926809-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7e0/9355642/20f3e952efde/fpls-13-926809-g007.jpg

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