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定位于液泡膜的硝酸盐摄取转运体参与拟南芥液泡中硝酸盐的外排和再分配。

Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis.

机构信息

National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, People's Republic of China.

University of Chinese Academy of Sciences, Beijing, 100049, China.

出版信息

Sci Rep. 2017 Jul 25;7(1):6417. doi: 10.1038/s41598-017-06744-5.

DOI:10.1038/s41598-017-06744-5
PMID:28743909
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5526873/
Abstract

A great proportion of nitrate taken up by plants is stored in vacuoles. Vacuolar nitrate accumulation and release is of great importance to nitrate reallocation and efficient utilization. However, how plants mediate nitrate efflux from vacuoles to cytoplasm is largely unknown. The current study identified NPF5.11, NPF5.12 and NPF5.16 as vacuolar nitrate efflux transporters in Arabidopsis. Histochemical analysis showed that NPF5.11, NPF5.12 and NPF5.16 were expressed preferentially in root pericycle cells and xylem parenchyma cells, and further analysis showed that these proteins were tonoplast-localized. Functional characterization using cRNA-injected Xenopus laevis oocytes showed that NPF5.11, NPF5.12 and NPF5.16 were low-affinity, pH-dependent nitrate uptake transporters. In npf5.11 npf5.12 npf5.16 triple mutant lines, more root-fed NO was translocated to shoots compared to the wild type control. In the NPF5.12 overexpression lines, proportionally less nitrate was maintained in roots. These data together suggested that NPF5.11, NPF5.12 and NPF5.16 might function to uptake nitrate from vacuoles into cytosol, thus serving as important players to modulate nitrate allocation between roots and shoots.

摘要

植物吸收的大量硝酸盐储存在液泡中。液泡中硝酸盐的积累和释放对硝酸盐的再分配和有效利用非常重要。然而，植物如何介导硝酸盐从液泡向细胞质的流出在很大程度上是未知的。本研究鉴定了拟南芥中的 NPF5.11、NPF5.12 和 NPF5.16 作为液泡硝酸盐外排转运蛋白。组织化学分析表明，NPF5.11、NPF5.12 和 NPF5.16 在根皮层细胞和木质部薄壁细胞中优先表达，进一步分析表明这些蛋白定位于液泡膜上。利用 cRNA 注射非洲爪蟾卵母细胞进行功能表征表明，NPF5.11、NPF5.12 和 NPF5.16 是低亲和力、依赖 pH 的硝酸盐摄取转运蛋白。在 npf5.11 npf5.12 npf5.16 三重突变体系中，与野生型对照相比，更多的根部供应的 NO 被转运到地上部分。在 NPF5.12 过表达系中，根中保持的硝酸盐比例减少。这些数据共同表明，NPF5.11、NPF5.12 和 NPF5.16 可能从液泡摄取硝酸盐进入细胞质，因此是调节根和地上部分之间硝酸盐分配的重要参与者。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/5da54745045b/41598_2017_6744_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/64cc83c8b7f7/41598_2017_6744_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/9af053841c23/41598_2017_6744_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/ff116a8b4bf7/41598_2017_6744_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/defcb734004c/41598_2017_6744_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/5da54745045b/41598_2017_6744_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/64cc83c8b7f7/41598_2017_6744_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/9af053841c23/41598_2017_6744_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/ff116a8b4bf7/41598_2017_6744_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/defcb734004c/41598_2017_6744_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/778f/5526873/5da54745045b/41598_2017_6744_Fig5_HTML.jpg

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