Zoology Department, College of Science, King Saud University, P.O. Box: 2455, 11451 Riyadh, Saudi Arabia.
Biomed Res Int. 2022 Jul 27;2022:6066567. doi: 10.1155/2022/6066567. eCollection 2022.
Cancer-testis (CT) genes are typically expressed in the testes; however, they have been linked to aberrant expression in a variety of malignancies. family genes are an example of CT genes. Therefore, the overarching objective of this study was to examine the expressions of family genes in several patients with colon cancer (CC) to see if they might be employed as cancer biomarkers in the early phases of cancer detection and to improve treatment. In this investigation, RT-PCR was used to analyze family genes in neighboring normal colon (NC) tissue from 10 CC patients. In addition, the effect of DNA demethylation on the expression status of the gene was evaluated by RT-PCR in HCT116 and Caco-2 cells and by qRT-PCR for HCT116 only after treating both CC cell lines with varying concentrations of 5-aza-2'-deoxycytidine (1.0, 5.0, and 10.0 M) for 48 or 72 hours. All family genes except for showed weak bands in several samples of NC tissues: , , , , and genes were observed in 40%, 50%, 40%, 30%, and 60% of the NC samples, respectively. Nonetheless, they had strong bands in multiple samples of CC tissues, with 70%, 90%, 60%, 50%, and 90% of the CC samples, respectively. Interestingly, was detected in 60% of CC tissues but not in NC tissues, suggesting that it is a potential biomarker for early CC detection. expression was not observed in either untreated or DMSO-treated HCT116 cells after 48 or 72 hours of treatment. However, according to the RT-PCR and qRT-PCR results, the gene was overexpressed in the HCT116 cells treated with three different concentrations of 5-aza-2'-deoxycytidine. This shows that demethylation plays a crucial role in expression activation.
癌症睾丸（CT）基因通常在睾丸中表达；然而，它们与多种恶性肿瘤中的异常表达有关。 家族基因就是 CT 基因的一个例子。因此，本研究的总体目标是检查几种结肠癌（CC）患者中 家族基因的表达情况，以确定它们是否可以作为癌症早期检测的生物标志物，并改善治疗效果。在这项研究中，我们使用 RT-PCR 分析了 10 例 CC 患者的相邻正常结肠（NC）组织中的 家族基因。此外，我们还通过 RT-PCR 评估了 DNA 去甲基化对 HCT116 和 Caco-2 细胞中 基因表达状态的影响，仅对 HCT116 细胞进行了 qRT-PCR 检测，并用不同浓度的 5-氮杂-2′-脱氧胞苷（1.0、5.0 和 10.0 μM）处理两种 CC 细胞系 48 或 72 小时后，仅对 HCT116 细胞进行了 qRT-PCR 检测。除 基因外，所有家族基因在几个 NC 组织样本中均显示出较弱的条带： 、 、 、 和 基因分别在 40%、50%、40%、30%和 60%的 NC 样本中观察到。然而，它们在多个 CC 组织样本中均有较强的条带，分别在 70%、90%、60%、50%和 90%的 CC 样本中观察到。有趣的是， 基因在 60%的 CC 组织中检测到，但在 NC 组织中未检测到，这表明它是早期 CC 检测的潜在生物标志物。未观察到未经处理或 DMSO 处理的 HCT116 细胞在 48 或 72 小时后表达 基因。然而，根据 RT-PCR 和 qRT-PCR 的结果，在 HCT116 细胞中用三种不同浓度的 5-氮杂-2′-脱氧胞苷处理后， 基因过度表达。这表明去甲基化在 基因激活中起着关键作用。
