School of Nursing, Yangzhou University, Yangzhou 225000, PR China.
College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
Analyst. 2022 Sep 12;147(18):4049-4054. doi: 10.1039/d2an01003h.
In this research, dual-strategy biosensing of glucose was proposed based on multifunctional CuWO nanoparticles (CuWO NPs), which were prepared for the application of electrochemical and colorimetric sensing of glucose. CuWO NPs show large specific surface area and good conductivity as well as excellent peroxidase-like activity. A sensitive and selective electrochemical glucose biosensor was fabricated with the immobilization of glucose oxidase (GOx) on a CuWO NP modified electrode for enhancing the direct electron transfer behavior. A wide linear range of 0.005-1.8 mM with a low detection limit of 1.5 μM and a high sensitivity of 28.02 mA M cm were achieved by using the electrochemical biosensor. Meanwhile, a colorimetric and visual glucose biosensor was constructed based on the GOx/CuWO cascade nanozyme, which shows a linear range of 0.05-1.0 mM with excellent selectivity. CuWO NPs as a promising matrix open up a dual-strategy biosensor for sensitive and selective detection of glucose.
在这项研究中，提出了基于多功能 CuWO 纳米粒子（CuWO NPs）的葡萄糖双策略生物传感，该纳米粒子用于葡萄糖的电化学和比色传感应用。CuWO NPs 具有大的比表面积和良好的导电性以及优异的过氧化物酶样活性。通过将葡萄糖氧化酶（GOx）固定在 CuWO NP 修饰电极上，制备了灵敏和选择性的电化学葡萄糖生物传感器，以增强直接电子转移行为。电化学生物传感器实现了宽线性范围为 0.005-1.8 mM，检测限低至 1.5 μM，灵敏度高至 28.02 mA M cm。同时，基于 GOx/CuWO 级联纳米酶构建了比色和可视化葡萄糖生物传感器，其线性范围为 0.05-1.0 mM，具有优异的选择性。CuWO NPs 作为一种有前途的基质，为葡萄糖的灵敏和选择性检测开辟了一种双策略生物传感器。
