School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, China.
J Biomed Mater Res B Appl Biomater. 2023 Jan;111(1):173-183. doi: 10.1002/jbm.b.35143. Epub 2022 Aug 8.
In this research, bioactive glass particles prepared by the reactive flash nanoprecipitation method (RFNP-BG particles) are used to crosslink sodium alginate to prepare biological sponges (SA-BG sponges) by freeze-drying. An experiment for the cross-linking mechanism confirms that the continuous release of Ca from RFNP-BG is promoted by the crosslinking reaction and in turn leads to the gelation process of SA. Bioactive glass particles not only provide Ca for the crosslinking of sodium alginate, but also enhance the mechanical properties of the SA-BG sponges. The results show that the elastic modulus of the SA-BG sponges increases from 0.026 MPa to 0.641 MPa, and the resistance to external force deformation is greatly improved; the thermal decomposition temperature increases from 105°C to 166°C; compared with a pure SA sponge, the water resistance is significantly improved. In vitro cell experiments show that the SA-BG sponges have a certain adverse effect on cell proliferation, but it is in an acceptable range. qPCR results show that the SA-BG sponges have a certain beneficial effect on promoting osteogenic gene expression. The SA-BG sponges have great application potential in the fields of medicine, hemostasis, and wound closure.
在这项研究中，使用反应闪蒸纳米沉淀法（RFNP-BG 颗粒）制备的生物活性玻璃颗粒来交联海藻酸钠，通过冷冻干燥制备生物海绵（SA-BG 海绵）。交联机制的实验证实，RFNP-BG 的连续 Ca 释放受到交联反应的促进，进而导致 SA 的胶凝过程。生物活性玻璃颗粒不仅为海藻酸钠的交联提供 Ca，而且还增强了 SA-BG 海绵的机械性能。结果表明，SA-BG 海绵的弹性模量从 0.026 MPa 增加到 0.641 MPa，对外力变形的抵抗力大大提高；热分解温度从 105°C 增加到 166°C；与纯 SA 海绵相比，耐水性显著提高。体外细胞实验表明，SA-BG 海绵对细胞增殖有一定的不利影响，但在可接受的范围内。qPCR 结果表明，SA-BG 海绵对促进成骨基因表达具有一定的有益作用。SA-BG 海绵在医学、止血和伤口闭合等领域具有巨大的应用潜力。
