Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China; Hunan Engineering Technology Research Center for Comprehensive Development and Utilization of Biomass Resources, College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425199, China.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China.
Int J Biol Macromol. 2023 Apr 1;233:123532. doi: 10.1016/j.ijbiomac.2023.123532. Epub 2023 Feb 3.
There are several factors that contribute to the mortality of people who suffer from unmanageable bleeding. Therefore, the development of rapid hemostatic materials is necessary. Herein, novel rapid hemostatic composite sponges were developed by incorporation of kaolin (K) into carboxymethyl chitosan (CMCS)/sodium alginate (SA) via a combination of methods that includes ionic crosslinking, polyelectrolyte action, and freeze-drying. The CMCS/SA-K composite sponges were cross-linked with calcium ions provided by a sustained-release system consisting of D-gluconolactone (GDL) and Ca-EDTA, and the hemostatic ability of the sponges was enhanced by loading the inorganic hemostatic agent-kaolin (K). It was demonstrated that the CMCS/SA-K composite sponges had a good porous structure and water absorption properties, excellent mechanical properties, outstanding biodegradability, and biocompatibility. Simultaneously, they exhibited rapid hemostatic properties, both in vitro and in vivo. Significantly, the hemostatic time of the CMCS/SA-K sponge was improved by 82.76 %, 191.82 %, and 153.05 %, compared with those of commercially available gelatin sponges in the rat tail amputation, femoral vein, and liver injury hemorrhage models respectively, indicating that its hemostatic ability was superior to that of commercially available hemostatic materials. Therefore, CMCS/SA-K composite sponges show great promise for rapid hemostasis.
有几个因素导致难以控制出血的患者死亡率高。因此，有必要开发快速止血材料。本文通过离子交联、聚电解质作用和冷冻干燥相结合的方法，将高岭土（K）掺入羧甲基壳聚糖（CMCS）/海藻酸钠（SA）中，开发了新型快速止血复合海绵。CMCS/SA-K 复合海绵通过由 D-葡萄糖酸内酯（GDL）和 Ca-EDTA 组成的缓释系统提供的钙离子交联，通过加载无机止血剂-高岭土（K）提高了海绵的止血能力。结果表明，CMCS/SA-K 复合海绵具有良好的多孔结构和吸水性、优异的力学性能、出色的生物降解性和生物相容性。同时，它们在体外和体内均表现出快速止血性能。值得注意的是，与市售明胶海绵相比，CMCS/SA-K 海绵在大鼠尾切断、股静脉和肝损伤出血模型中的止血时间分别提高了 82.76%、191.82%和 153.05%，表明其止血能力优于市售止血材料。因此，CMCS/SA-K 复合海绵在快速止血方面具有广阔的应用前景。
