Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058, Erlangen, Germany.
Institute of Polymer Materials, University of Erlangen-Nuremberg, Martensstr. 7, 91058, Erlangen, Germany.
Macromol Biosci. 2020 Oct;20(10):e2000138. doi: 10.1002/mabi.202000138. Epub 2020 Aug 17.
The effect of the incorporation of 45S5 bioactive glass (BG) microparticles (mean particle size ≈ 2 µm) on the fabrication and physicochemical properties of alginate dialdehyde-gelatin hydrogel capsules is investigated. The addition of BG particles decreases the hydrogel gelation time by ≈79% and 91% for the samples containing 0.1% w/v and 0.5% w/v BG, respectively. Moreover, it results in increasing average diameter of hydrogel capsules produced via a pressure-driven extrusion technique from about 1000 µm for the samples without BG to about 1700 and 1900 µm for the samples containing BG at concentrations of 0.1% w/v and 0.5% w/v, respectively. The presence of BG particles in the capsules decreases the degradation rate and improves the bioactivity of the materials. The viability of MG-63 cells encapsulated in all samples increases during the first 7 d of cultivation and maintains the same level during 21 d of cultivation. The early cell viability in samples containing BG is lower than that in samples without BG. The results show that 45S5 BG can positively regulate the osteogenic activity of cells incorporated in hydrogel capsules. The fabricated composite capsules exhibit promising potential for cell delivery in bone regeneration applications.
研究了 45S5 生物活性玻璃（BG）微颗粒（平均粒径约为 2μm）的掺入对海藻酸钠乙二醛-明胶水凝胶胶囊的制备和物理化学性质的影响。BG 颗粒的添加分别使含 0.1%w/v 和 0.5%w/v BG 的样品的水凝胶凝胶时间缩短了约 79%和 91%。此外，它导致通过压力驱动挤出技术生产的水凝胶胶囊的平均直径从不含 BG 的样品的约 1000μm 分别增加到含 0.1%w/v 和 0.5%w/v BG 的样品的约 1700μm 和 1900μm。BG 颗粒在胶囊中的存在降低了降解速率并提高了材料的生物活性。在培养的前 7 天，所有样品中封装的 MG-63 细胞的活力增加，并在培养的 21 天内保持相同水平。含 BG 样品的早期细胞活力低于不含 BG 的样品。结果表明，45S5 BG 可以积极调节水凝胶胶囊中细胞的成骨活性。所制备的复合胶囊在骨再生应用中的细胞递送方面表现出很大的潜力。
