LAQV-REQUIMTE, Laboratory of Bromatology and Hydrology, Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal.
UCIBIO, REQUIMTE, Biological Sciences Department, Laboratory of Biochemistry, Faculty of Pharmacy, University of Porto, Porto, Portugal; Associate Laboratory i4HB - Institute for Health and Bioeconomy - Faculty of Pharmacy, University of Porto, Rua Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal.
Toxicology. 2022 Aug;478:153282. doi: 10.1016/j.tox.2022.153282. Epub 2022 Aug 6.
Disruption of non-differentiated endometrial stromal cells could have noxious consequences in female reproduction, impairing endometrial remodelling and implantation. Following the classification of bisphenol A (BPA) as an endocrine disrupting chemical, it started to be gradually withdrawn from the market, being substituted by structural analogues, whose effects in human health are not fully understood. This work used a telomerase-immortalized human endometrial stromal cell line (St-T1b) to study the effects of BPA and its three most commercialized structural analogues (ranked: bisphenols S, F and AF) on endometrial stromal cells to understand their effects on female reproductive function. Bisphenols showed dissimilar effects. All four compounds generated endoplasmic reticulum (ER) stress. In addition, bisphenols A, F and AF induced apoptosis through different mechanisms, with bisphenol AF causing cell cycle arrest at G/M phase. Bisphenol AF decreased mitochondrial transmembrane potential and bisphenols A, F and AF produced oxidative stress.
未分化的子宫内膜基质细胞的破坏可能对女性生殖系统造成有害影响，损害子宫内膜重塑和着床。双酚 A（BPA）被归类为内分泌干扰化学物质后，开始逐渐从市场上撤出，被结构类似物所取代，而这些类似物对人类健康的影响尚未完全了解。本研究使用端粒酶永生化的人子宫内膜基质细胞系（St-T1b）来研究 BPA 及其三种最商业化的结构类似物（排名：双酚 S、F 和 AF）对子宫内膜基质细胞的影响，以了解它们对女性生殖功能的影响。双酚表现出不同的作用。这四种化合物都产生了内质网（ER）应激。此外，双酚 A、F 和 AF 通过不同的机制诱导细胞凋亡，而双酚 AF 导致细胞周期在 G/M 期停滞。双酚 AF 降低了线粒体跨膜电位，而双酚 A、F 和 AF 则产生了氧化应激。
