Dumoulin Alexandre, Stoeckli Esther T
Department of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
Department of Molecular Life Sciences and Neuroscience Center Zurich, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
Neuroscience. 2023 Jan 1;508:30-39. doi: 10.1016/j.neuroscience.2022.08.005. Epub 2022 Aug 6.
The molecular mechanisms of neural circuit formation have been of interest to Santiago Ramón y Cajal and thousands of neuroscientists sharing his passion for neural circuits ever since. Cajal was a brilliant observer and taught us about the connections and the morphology of neurons in the adult and developing nervous system. Clearly, we will not learn about molecular mechanisms by just looking at brain sections or cells in culture. Technically, we had to come a long way to today's possibilities that allow us to perturb target gene expression and watch the consequences of our manipulations on navigating axons in situ. In this review, we summarize landmark steps towards modern live-imaging approaches used to study the molecular basis of axon guidance.
自圣地亚哥·拉蒙·伊·卡哈尔时代起，神经回路形成的分子机制就一直吸引着他以及数千名和他一样对神经回路充满热情的神经科学家。卡哈尔是一位杰出的观察者，他向我们揭示了成年及发育中神经系统中神经元的连接方式和形态。显然，仅仅观察脑切片或培养中的细胞，我们无法了解分子机制。从技术层面讲，我们历经漫长的道路才发展到如今能够干扰靶基因表达，并观察我们的操作对轴突在原位导航的影响。在这篇综述中，我们总结了用于研究轴突导向分子基础的现代活体成像方法所取得的具有里程碑意义的进展。
