Brain Research Institute, University of Zurich, Zurich, Switzerland.
University Research Priority Program (URPP), Adaptive Brain Circuits in Development and Learning, University of Zurich, Zurich, Switzerland.
Nat Commun. 2024 Mar 27;15(1):2679. doi: 10.1038/s41467-024-46770-2.
In 2015, we launched the mesoSPIM initiative, an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, the demand for imaging larger samples at higher speed and resolution has increased, requiring major improvements in the capabilities of such microscopes. Here, we introduce the next-generation mesoSPIM ("Benchtop") with a significantly increased field of view, improved resolution, higher throughput, more affordable cost, and simpler assembly compared to the original version. We develop an optical method for testing detection objectives that enables us to select objectives optimal for light-sheet imaging with large-sensor cameras. The improved mesoSPIM achieves high spatial resolution (1.5 µm laterally, 3.3 µm axially) across the entire field of view, magnification up to 20×, and supports sample sizes ranging from sub-mm up to several centimeters while being compatible with multiple clearing techniques. The microscope serves a broad range of applications in neuroscience, developmental biology, pathology, and even physics.
2015 年，我们启动了 mesoSPIM 计划，这是一个开源项目，旨在使大组织透明化的光片显微镜更易于使用。同时，对更大样本以更高速度和分辨率进行成像的需求增加，这需要此类显微镜的能力有重大改进。在这里，我们介绍了下一代 mesoSPIM（“台式机”），与原始版本相比，它具有更大的视野、更高的分辨率、更高的吞吐量、更实惠的成本和更简单的组装。我们开发了一种用于测试检测目标的光学方法，使我们能够为具有大传感器相机的光片成像选择最佳的目标。改进后的 mesoSPIM 在整个视场中实现了高空间分辨率（横向 1.5 µm，轴向 3.3 µm）、高达 20 倍的放大率，并支持从亚毫米到几厘米的样品尺寸，同时兼容多种透明化技术。该显微镜适用于神经科学、发育生物学、病理学，甚至物理学等广泛的应用领域。
