Academy of National Food and Strategic Reserves Administration, Beijing 100037, PR China.
Academy of National Food and Strategic Reserves Administration, Beijing 100037, PR China.
Food Res Int. 2022 Sep;159:111511. doi: 10.1016/j.foodres.2022.111511. Epub 2022 Jun 14.
Wuchang Daohuaxiang (DHX) rice is a famous product in China, while identification and authentication method is limited. Due to the special taste and flavor of DHX, lipidomics was conducted to discriminate it from faked rice. First, lipid profiles of DHX and other nine rice varieties were required from the established UPLC-Q/TOF-MS/MS method. The compositions and contents of lipids significantly varied among different rice. Then, twenty-six potential biomarkers containing 6 DGs, 5 PAs, 4 PCs, 1 PE, 3 PGs, 2 PIs, 2 PSs, 2 SMs, and 1 Cer were characterized and verified for identifying DHX from all faked rice. The accuracy of developed methods for DHX discrimination was 97.14%. In addition, OPLS-DA models were established for DHX distinction between each of the nine rice cultivars. Our results would supply as supplementary tools for discrimination of DHX rice for supervision departments and industries.
武昌稻花香(DHX)米是中国的名优产品,但鉴定和认证方法有限。由于 DHX 具有特殊的口感和风味,因此采用脂质组学方法将其与假冒大米区分开来。首先,需要从建立的 UPLC-Q/TOF-MS/MS 方法中获得 DHX 和其他九种大米品种的脂质图谱。不同大米的脂质组成和含量存在显著差异。然后,鉴定出 26 种潜在的生物标志物,其中含有 6 种二酰基甘油(DG)、5 种溶血磷脂酰胆碱(PC)、4 种磷脂酰乙醇胺(PE)、3 种磷脂酰甘油(PG)、2 种磷脂酰肌醇(PI)、2 种鞘磷脂(SM)和 1 种神经酰胺(Cer),用于从所有假冒大米中鉴别 DHX。开发的用于 DHX 鉴别方法的准确率为 97.14%。此外,还建立了 OPLS-DA 模型,用于区分九种大米品种中的 DHX。我们的研究结果将为监管部门和行业提供鉴别 DHX 大米的辅助工具。