National Demonstration Center for Experimental Ethnopharmacology Education, School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, P. R. China.
State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Bejing, P. R. China.
J Sci Food Agric. 2023 Jan 30;103(2):837-845. doi: 10.1002/jsfa.12195. Epub 2022 Sep 15.
Chrysanthemum is one of the most important and popular ornamentals over the world. Chrysanthemum drink is a type of traditional healthy drink like Chinese tea. Owing to the differences in the chemical compositions, different chrysanthemum varieties have different medicinal effects on human health. Thus, the identification of different chrysanthemum varieties is very important and necessary. This study aims to distinguish seven chrysanthemum varieties that are widely used in China. First, total lipids were obtained from chrysanthemums. After that, lipid profiles were characterized using ultra-high-performance liquid chromatography hyphenated with a Q Exactive™ high resolution-accurate-mass mass spectrometer.
A total of 163 lipid molecular species from 17 types of lipid classes in seven varieties of chrysanthemums were determined. Principal component analysis indicated that three lipid molecules, lysophosphatidylethanolamine(18:2) (LPE(18:2)), LPE(16:0), and phosphatidic acid(18:2/18:3) (variable importance in projection >3, P < 0.001), can be used as potential biomarkers to distinguish seven chrysanthemum varieties. Hierarchical cluster analysis showed that the lipid molecular profiles of 'Gongju' were most similar to 'Jinzijianju', followed by 'Huaibaiju', 'Boju', 'Hangbaiju', 'Chuju', and 'Fubaiju'.
This comprehensive analysis provided a new method to identify chrysanthemum varieties through the perspective of lipidomics combined with chemometrics. © 2022 Society of Chemical Industry.
菊花是世界上最重要和最受欢迎的观赏植物之一。菊花饮料是一种类似于中国茶的传统保健饮品。由于化学成分的差异，不同品种的菊花对人体健康有不同的药用功效。因此，鉴别不同品种的菊花非常重要和必要。本研究旨在鉴别中国广泛使用的 7 种菊花品种。首先，从菊花中提取总脂质。然后，使用超高效液相色谱- Q Exactive ™高分辨率-精确质量质谱联用仪对脂质图谱进行特征描述。
从 7 种菊花的 17 种脂质类型中确定了 163 种脂质分子种。主成分分析表明，三种脂质分子，溶血磷脂酰乙醇胺（18:2）（LPE（18:2））、LPE（16:0）和磷脂酸（18:2/18:3）（投影变量重要性>3，P <0.001），可用作潜在的生物标志物来区分 7 种菊花品种。层次聚类分析表明，“贡菊”的脂质分子图谱与“金盏菊”最为相似，其次是“白芍菊”、“博菊”、“杭白菊”、“滁菊”和“福白菊”。
本综合分析为通过脂质组学与化学计量学相结合的方法鉴别菊花品种提供了一种新方法。 © 2022 化学工业协会。
