IRTA-Food Safety and Functionality Programme. Finca Camps i Armet s/n, 17121 Monells, Girona, Spain.
Spanish Institute of Oceanography of the Spanish National Research Council (IEO, CSIC), Oceanographic Centre of Santander, Marine Culture Units "El Bocal", Seaweeds Centre. Barrio Corbanera s/n., 39012 Monte, Santander, Spain.
Food Res Int. 2022 Sep;159:111609. doi: 10.1016/j.foodres.2022.111609. Epub 2022 Jul 8.
Current trends towards the use of ingredients from natural origin in food, cosmetic and pharmaceutical industry, place macroalgae as a good reservoir of novel compounds. Among them, lipophilic major pigments such as chlorophylls and fucoxanthin, are of great interest because of their multiple applications as bioactive compounds and dyes. In this work, a mid-polarity medium was used to extract pigments from twenty-four species from North coast of Spain, including brown (Phaeophyceae) and red macroalgae (Rhodophyta). The fucoxanthin and chlorophyll a content was assessed by means of two different methods, spectrophotometric and high-performance liquid chromatography coupled to diode array detection (HPLC-DAD). The effect of dried processing on the pigment content of selected species was also evaluated. A linear relationship between the extractability of fucoxanthin and chlorophyll a was observed, being the highest content recorded among members belonging to the order Fucales and Undaria pinnatifida. This work provides good insights about the content on pigments in Spanish North Atlantic macroalgae with future commercial value in different industrial fields, as well as a critical overview of the suitability of the quantification methods and challenges related to their effect in results evaluation.
目前，在食品、化妆品和制药行业中，使用天然来源成分的趋势越来越明显，这使得大型藻类成为新型化合物的良好来源。其中，脂溶性主要色素如叶绿素和岩藻黄素，由于其作为生物活性化合物和染料的多种应用而备受关注。在这项工作中，使用中等极性介质从西班牙北海岸的 24 种物种中提取色素，包括褐藻（Phacophyceae）和红藻（Rhodophyta）。通过两种不同的方法，分光光度法和高效液相色谱法与二极管阵列检测（HPLC-DAD）来评估岩藻黄素和叶绿素 a 的含量。还评估了干燥处理对选定物种色素含量的影响。观察到岩藻黄素和叶绿素 a 的提取率之间存在线性关系，在属于 Fucales 目和裙带菜属的成员中记录到的含量最高。这项工作提供了关于西班牙北大西洋大型藻类中色素含量的深入了解，这些藻类具有未来在不同工业领域的商业价值，同时还对定量方法的适用性以及它们对结果评估的影响进行了批判性概述。
