去甲肾上腺素对从豚鼠肺动脉新鲜分离的单个平滑肌细胞的作用。
The action of noradrenaline on single smooth muscle cells freshly dispersed from the guinea-pig pulmonary artery.
作者信息
Byrne N G, Large W A
出版信息
Br J Pharmacol. 1987 May;91(1):89-94. doi: 10.1111/j.1476-5381.1987.tb08986.x.
Abstract
Responses to ionophoretically-applied noradrenaline were investigated with micro-electrodes in whole tissue preparations and with patch pipettes in isolated cells dispersed from the guinea-pig pulmonary artery. In whole tissue and dispersed cells noradrenaline evoked monophasic depolarizations which had a similar time course. In dispersed cells the amplitude of electronic potentials was reduced during the noradrenaline-evoked depolarization. Under voltage clamp noradrenaline elicited an inward current, which persisted in 18 mM external potassium with the membrane potential set at the potassium equilibrium potential. In voltage clamp experiments the amplitude of current steps to hyperpolarizing voltage jumps was increased during the noradrenaline-induced inward current. These data suggest that the depolarization to noradrenaline in the guinea-pig pulmonary artery is mediated by an increase in membrane conductance.
摘要
在豚鼠肺动脉的全组织标本中，使用微电极研究了对离子电泳施加去甲肾上腺素的反应；在从豚鼠肺动脉分离出的单个细胞中，使用膜片吸管进行了研究。在全组织和分离细胞中，去甲肾上腺素均可诱发单相去极化，其时间进程相似。在分离细胞中，去甲肾上腺素诱发的去极化过程中，电紧张电位的幅度降低。在电压钳制下，去甲肾上腺素引发内向电流，当膜电位设定为钾平衡电位时，该电流在18 mM的细胞外钾中持续存在。在电压钳制实验中，去甲肾上腺素诱导的内向电流期间，向超极化电压跃变的电流阶跃幅度增加。这些数据表明，豚鼠肺动脉对去甲肾上腺素的去极化是由膜电导增加介导的。
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