Xi Jinfeng, Kan Wenjie, Zhu Yan, Huang Shengwei, Wu Lifang, Wang Jun
The Center for Ion Beam Bioengineering and Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China.
University of Science and Technology of China, Hefei 230026, Anhui, China.
Heliyon. 2022 Jul 29;8(8):e10021. doi: 10.1016/j.heliyon.2022.e10021. eCollection 2022 Aug.
Silver nanoparticles (AgNPs) synthesized from plant extracts have recently emerged as a rapidly growing field with numerous applications in pharmaceutical and clinical contexts. The purpose of this research is to come up with a novel method for the biosynthesis of silver nanoparticles that use leaf extract as a reducing agent. The synthesis of AgNPs was confirmed using UV-vis spectroscopy, and the properties of AgNPs were characterized using Transmission Electron Microscope, Fourier Infrared Spectrometer, X-ray diffraction, Thermogravimetric Analysis, and Zeta potential. The results showed that the AgNPs exhibited a characteristic absorption peak at 430 nm, their diameter ranged from 4 nm to 52 nm, and C, O, and Cl elements, which might represent flavonoids and phenolic components absorbed on the surface of AgNPs. The zeta potential of AgNPs was found to be -30.5 mV, which indicates repulsion among AgNPs and they have good dispersion stability. AgNPs have been found to suppress the tyrosinase activity both in mushroom tyrosinase and A375 cells, as well as diminish ROS formation in HaCat cells. According to this study, AgNPs is a novel material that can enhance skin health by preventing melanin development.
从植物提取物中合成的银纳米颗粒(AgNPs)最近已成为一个快速发展的领域,在制药和临床环境中有众多应用。本研究的目的是提出一种利用叶提取物作为还原剂生物合成银纳米颗粒的新方法。使用紫外可见光谱法确认了AgNPs的合成,并使用透射电子显微镜、傅里叶红外光谱仪、X射线衍射、热重分析和zeta电位对AgNPs的性质进行了表征。结果表明,AgNPs在430nm处呈现特征吸收峰,其直径范围为4nm至52nm,并且C、O和Cl元素可能代表吸附在AgNPs表面的黄酮类化合物和酚类成分。发现AgNPs的zeta电位为-30.5mV,这表明AgNPs之间存在排斥作用,并且它们具有良好的分散稳定性。已发现AgNPs在蘑菇酪氨酸酶和A375细胞中均能抑制酪氨酸酶活性,以及减少HaCat细胞中的活性氧生成。根据这项研究,AgNPs是一种新型材料,可以通过防止黑色素生成来促进皮肤健康。