Comparative study on larvicidal activity of green synthesized silver nanoparticles and (Annonaceae) aqueous extract to control and (Diptera: Culicidae).

The present study reports mosquito larvicidal potential of green synthesized silver nanoparticles by using leaves (An-AgNPs). Synthesized An-AgNPs were characterized by Ultraviolet-Visible spectroscopy (UV-VIS), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS) technique and Fourier transform infrared spectroscopy (FTIR). Colur change from pale yellow to brick red of the plant extract and AgNO solution indicated the formation of An-AgNPs initially. Surface Plasmon Resonance (SPR) band at 435 nm in the UV-Vis confirmed the formation of An-AgNPs. SEM images showed that An-AgNPs were spherical in shape. FTIR proved that An-AgNPs were functionalized with biomolecules in leaves. Based on DLS analysis the average size range of synthesized An-AgNPs was determine to be 10-100 nm and 100-1000 nm. Third instar larvae of dengue vector mosquitoes, and were subjected to larvicidal bioassays in a range of concentrations of An-AgNPs and crude aqueous leaf extract (2-10 mg/L). An-AgNPs exhibited very high larvicidal activity against dengue vector mosquito larvae; LC value for at 24 h exposure to An-AgNPs (Plant extract: AgNO 1 : 10) 5.29 mg/L; An-AgNPs (Plant extract: AgNO 2 : 10) 2.43 mg/L while LC value for at 24 h exposure to An-AgNPs (Plant extract: AgNO1:10) 3.02 mg/L; An-AgNPs (Plant extract: AgNO 2:10) 2.51 mg/L. LC values obtained for leaf extract tested against and are 5.94 mg/L and 5.00 mg/L respectively at 24-hour exposure. This study further revealed that . is more susceptible than to to a given concentration of An-AgNPs and to crude aqueous leaf extract of Larvicidal effect of An-AgNPs is superior to the crude aqueous leaf extract of . An-AgNPs is a potent larvicide for dengue vector control.