Girmaw Fentaw, Engidawork Ephrem
Department of Pharmacy, College of Medicine and Health Science, Woldia University, Woldia, Ethiopia.
Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, Addis Ababa University, Addis Ababa, Ethiopia.
Evid Based Complement Alternat Med. 2022 Jul 30;2022:2640648. doi: 10.1155/2022/2640648. eCollection 2022.
Drug resistance is a universal challenge to malaria control measures. As a result, the development and discovery of new chemotherapeutic agents from medicinal plants having anti-malarial traditional claims are very important. This work, therefore, attempted to evaluate the anti-malarial activity of the aqueous root extract of . using a rodent model of malaria.
The roots of . were extracted by hot decoction using distilled water. Anti-malarial activity of various doses (100 mg/kg, 200 mg/kg, and 600 mg/kg) of the root aqueous extract was evaluated using the 4-day suppressive test as well as curative and repository tests. Parasitemia, rectal temperature, body weight, PCV, and MST were also determined.
The finding showed that there were a dose-related significant parasitemia chemo-suppression and increment in survival time as compared to the negative control ( < 0.001) in all tests. The chemo-suppression effect was higher at 400 mg/kg extract-treated groups in the 4-day suppressive test followed by the curative test. The lowest chemo-prophylaxis effect was observed in 100 mg/kg extract-treated groups in the repository test. Regarding the other parameters, the extract prevented weight loss, temperature drop, and hemolysis in all models but not in a consistent manner.
The current study showed that the aqueous root extract of . possessed a varying degree of anti-malarial activity in all three tests, with greater parasitemia suppression observed in the 4-day suppressive test. The extract produced higher parasitemia chemo-suppression and longer survival time in early infections followed by established and then residual infection.
耐药性是疟疾控制措施面临的一个普遍挑战。因此，从具有抗疟传统说法的药用植物中开发和发现新的化学治疗剂非常重要。因此，这项工作试图使用疟疾啮齿动物模型评估[植物名称]根水提取物的抗疟活性。
[植物名称]的根用蒸馏水热煎煮提取。使用4天抑制试验以及治疗和储存试验评估根水提取物不同剂量（100mg/kg、200mg/kg和600mg/kg）的抗疟活性。还测定了寄生虫血症、直肠温度、体重、红细胞压积和平均存活时间。
研究结果表明，与阴性对照相比，在所有试验中均存在剂量相关的显著寄生虫血症化学抑制作用和存活时间增加（P<0.001）。在4天抑制试验中，400mg/kg提取物处理组的化学抑制作用较高，其次是治疗试验。在储存试验中，100mg/kg提取物处理组观察到最低的化学预防作用。关于其他参数，提取物在所有模型中均能防止体重减轻、体温下降和溶血，但并非始终如此。
目前的研究表明，[植物名称]根水提取物在所有三项试验中均具有不同程度的抗疟活性，在4天抑制试验中观察到更大的寄生虫血症抑制作用。该提取物在早期感染、随后的既定感染和残留感染中产生更高的寄生虫血症化学抑制作用和更长的存活时间。
