College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.
Genome. 2022 Nov 1;65(11):537-545. doi: 10.1139/gen-2022-0037. Epub 2022 Aug 9.
The 12-oxophytoeienoic acid reductase (OPR) is a kind of enzyme in the octadecanoid biosynthesis pathway that determines the biosynthesis of jasmonic acid. Although the roles of OPRs have been extensively studied in several crop plants, little is known about the biological functions of OPR-encoding genes in plants. In this study, seven OPR family genes () were identified from the genome. The physical and chemical properties of were further analyzed, including gene expression patterns, promoter elements, and chromosomal locations. The results showed that the seven CaOPR homologues could be divided into two subgroups, and CaOPR6 was highly similar to AtOPR3 in . The expression of was significantly induced by various stresses such as cold, salt, and pathogen infection, indicating that plays important roles in response to abiotic and biotic stresses. Overall, these findings improve the understanding of the biological functions of in the development of pepper fruit and stress response of pepper plants, and facilitate further studies on the molecular biology of OPR proteins in vegetables.
12-氧代植烯酸还原酶（OPR）是十八碳类生物合成途径中的一种酶，决定了茉莉酸的生物合成。尽管 OPR 在几种作物中的作用已得到广泛研究，但关于植物中 OPR 编码基因的生物学功能知之甚少。在这项研究中，从 基因组中鉴定出了 7 个 OPR 家族基因。进一步分析了 的理化性质，包括基因表达模式、启动子元件和染色体位置。结果表明，这 7 个 CaOPR 同源物可以分为两个亚组，CaOPR6 与 中的 AtOPR3 高度相似。 的表达受到冷、盐和病原体感染等各种胁迫的显著诱导，表明 在非生物和生物胁迫响应中发挥重要作用。总的来说，这些发现提高了我们对 在辣椒果实发育和辣椒植株胁迫响应中的生物学功能的理解，并促进了对 OPR 蛋白在蔬菜中分子生物学的进一步研究。
