在牙科医院环境中，使用酶清洁剂和自动化清洗消毒器对多个高速手机的内部气路和水路、机头和机头等部件进行有效清洁和消毒。

Effective cleaning and decontamination of the internal air and water channels, heads and head-gears of multiple contra-angle dental handpieces using an enzymatic detergent and automated washer-disinfection in a dental hospital setting.

机构信息

University of Dublin Trinity College, Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, Dublin, Ireland.

Central Sterile Services Department, Dublin Dental University Hospital, Dublin, Ireland.

出版信息

J Hosp Infect. 2022 Oct;128:80-88. doi: 10.1016/j.jhin.2022.07.019. Epub 2022 Aug 6.

DOI:10.1016/j.jhin.2022.07.019

PMID:35944787

Abstract

BACKGROUND

Dental handpieces (DHPs) are reusable invasive medical devices that must be cleaned, decontaminated, lubricated and steam sterilized after use. DHPs have a complex internal design including narrow channels, contamination of which can compromise sterilization. DHPs are not designed for routine disassembly, making cleaning/decontamination efficacy difficult to monitor. Washer-disinfection is the preferred method of decontaminating DHPs, but few studies have investigated its direct effectiveness at reducing microbial contamination internally.

AIMS

To use contra-angle DHPs as a model system to investigate the effectiveness of washer-disinfection at reducing microbial contamination of internal components of multiple DHPs.

METHODS

The air and water channels and heads of 10 disassembled contra-angle DHPs (BienAir, Biel/Bienne, Switzerland) were inoculated separately with 10 colony forming units (cfu) of Pseudomanas aeruginosa, Staphylococcus aureus, Enterococcus hirae or Candida albicans in the presence of 0.3% bovine serum albumin (BSA) (clean conditions), 3.0% BSA or 10% artificial test soil (dirty conditions). After reassembly, all 10 DHPs underwent washer-disinfection simultaneously in a Míele (Míele Ireland Ltd., Dublin, Ireland) PG8528 washer-disinfector and were tested for reductions in micro-organisms and protein. Additional experiments were undertaken with three lubricated DHPs inoculated with S. aureus and 10% test soil. All experiments were repeated in triplicate.

FINDINGS

On average, an approximate 5 log or greater reduction in microbial cfu and a >93% reduction in protein from DHP heads and channels was consistently recorded following washer-disinfection for all DHPs under all conditions tested.

CONCLUSIONS

The internal components of multiple DHPs can be effectively cleaned and decontaminated by washer-disinfection.

摘要

背景

牙科机头（DHPs）是可重复使用的侵入性医疗器械，使用后必须进行清洗、消毒、润滑和蒸汽灭菌。DHPs 具有复杂的内部设计，包括狭窄的通道，其污染会影响消毒效果。DHPs 并非专为常规拆卸而设计，因此难以监测清洁/消毒效果。清洗消毒是消毒 DHP 的首选方法，但很少有研究调查其在减少内部微生物污染方面的直接效果。

目的

使用角向机头作为模型系统，研究清洗消毒在减少多种 DHP 内部部件微生物污染方面的有效性。

方法

将 10 个拆开的角向机头（BienAir，Biel/Bienne，瑞士）的空气和水通道以及机头分别用 10 个假单胞菌、金黄色葡萄球菌、屎肠球菌或白色念珠菌的 10 个菌落形成单位（cfu）接种，同时存在 0.3%牛血清白蛋白（BSA）（清洁条件）、3.0%BSA 或 10%人工测试土壤（污染条件）。重新组装后，将所有 10 个 DHP 同时放入 Míele（Míele Ireland Ltd.，都柏林，爱尔兰）PG8528 清洗消毒器中进行清洗消毒，并检测微生物和蛋白质的减少量。另外还对 3 个涂有润滑剂的机头进行了接种金黄色葡萄球菌和 10%测试土壤的实验。所有实验均重复三次。