Henan University of Science and Technology, Luoyang, 471000, China; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science (CAFS), Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology, Qingdao, 266071, China.
Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals, Guangdong Ocean University, Zhanjiang, 524088, China.
Gene Expr Patterns. 2022 Sep;45:119265. doi: 10.1016/j.gep.2022.119265. Epub 2022 Aug 6.
Patched (Ptch) is a receptor in the hedgehog signaling pathway, essential for animal development. Our previous study showed that ptch1 gene participates in the maintenance of the male germline and spermatogenesis in Cynoglossus semilaevis (csptch1). In this study, we identified a patched1 gene homolog (csptch1 x1). The csptch1 x1 gene is 5761 bp long, with a 4638 bp coding sequence that encodes 1545 amino acids. The Csptch1 x1 protein has 12 transmembrane regions and sterol-sensing domains and is highly homologous to the csptch1 (91 amino acids difference). Expression pattern analysis showed that csptch1 x1 is expressed in eight different tissues of adult tongue sole, and the expression is significantly higher in tissues of female than that in male tissues. The expression pattern in developmental stages was also analyzed. csptch1 x1 could be detected at the 1-cell stage and was highly expressed at the blastocyst, somite, and blastopore closing stages, implying that it participates in cell differentiation. In ovarian development, the expression of csptch1 x1 was initiated at 20 days after hatching (dah) and was significantly high at 35-50 and 95-150 dah. In situ hybridization showed that csptch1 x1 was predominantly expressed in primordial germ cells, oocytes, and follicular cells, but the expression of the gene was lower in the testis. These results suggest that csptch1 x1 may be mainly involved in female differentiation and ovarian development, different from the role of csptch1 in spermatogenesis.
patched（Ptch）是 hedgehog 信号通路中的一种受体，对动物发育至关重要。我们之前的研究表明，ptch1 基因参与了泥鳅 Cynoglossus semilaevis（csptch1）的精原细胞和精子发生的维持。在本研究中，我们鉴定了 patched1 基因的同源物（csptch1 x1）。csptch1 x1 基因长 5761bp，具有 4638bp 的编码序列，编码 1545 个氨基酸。Csptch1 x1 蛋白具有 12 个跨膜区和固醇感应结构域，与 csptch1 高度同源（91 个氨基酸差异）。表达模式分析表明，csptch1 x1 在成年舌鳎的 8 种不同组织中表达，在雌性组织中的表达显著高于雄性组织。还分析了发育阶段的表达模式。csptch1 x1 可在 1 细胞期检测到，在囊胚、体节和胚孔关闭阶段高度表达，表明其参与了细胞分化。在卵巢发育过程中，csptch1 x1 的表达于孵化后 20 天（dah）开始，在 35-50 和 95-150 dah 时显著升高。原位杂交显示，csptch1 x1 主要在原始生殖细胞、卵母细胞和滤泡细胞中表达，但在睾丸中表达较低。这些结果表明，csptch1 x1 可能主要参与雌性分化和卵巢发育，与 csptch1 在精子发生中的作用不同。
